@article{Banović2021c,
title = {A One Health approach to study the circulation of tick-borne pathogens: A preliminary study},
author = {Pavle Banović and Adrian Alberto Díaz-Sánchez and Clemence Galon and Angélique Foucault-Simonin and Verica Simin and Dragana Mijatović and Luka Papić and Alejandra Wu-Chuang and Dasiel Obregon and Sara Moutailler and Alejandro Cabezas-Cruz },
doi = {https://doi.org/10.1016/j.onehlt.2021.100270},
year = {2021},
date = {2021-06-01},
journal = {One Health},
abstract = {Tick-borne pathogens (TBPs) have complex life cycles involving tick vectors and vertebrate hosts. However, there is limited empirical evidence on the zoonotic circulation of TBPs. In this study, we used a One Health approach to study the possible circulation of TBPs in ticks, animals and humans within a rural household in the foothills of the Fruška Gora mountain, northern Serbia. The presence of TBP DNA was assessed using microfluidic PCR (25 bacterial species, 7 parasite species, 5 bacterial genera, 3 parasite genera) in animal, human and tick samples and the presence of tick-borne encephalitis virus (TBEV) RNA was screened for using RT-qPCR on tick samples. In addition, Lyme borreliosis serology was assessed in patient sera. Rhipicephalus sanguineus and Ixodes ricinus ticks were identified on dogs and Haemaphysalis punctata was identified on house walls. Rickettsia helvetica was the most common pathogen detected in pooled R. sanguineus and I. ricinus tick samples, followed by Hepatozoon canis. None of the H. punctata tick samples tested positive for the presence of TBPs. Anaplasma phagocytophilum and Rickettsia monacensis were the most frequent pathogens detected in dogs, followed by Rickettsia felis, whereas Anaplasma bovis was the only pathogen found in one of the goats tested. None of the human blood samples collected from family members tested positive for the presence of TBPs. Although microfluidic PCR did not detect Borrelia sp. in any of the tested tick or blood samples, a family member with a history of Lyme disease was seropositive for Borrelia burgdorferi sensu lato (s.l.). We conclude that, despite the presence of TBPs in tick and vertebrate reservoirs, there is no evidence of infection with TBPs across various components of the epidemiological chain in a rural Fruška Gora household.},
keywords = {},
pubstate = {published},
tppubtype = {article}
}

@article{Ristić2021,
title = {Uticaj hroničnog imobilizacionog stresa na estrusni ciklus kod ženki miševa soja NMRI},
author = {Jelena Ristić and Damir Bogdan and Pavle Banović},
doi = {10.5937/mp72-28778},
year = {2021},
date = {2021-05-20},
journal = {Medicinski podmladak},
volume = {72},
number = {1},
pages = {6-11},
abstract = {Uvod: Stres je skup reakcija organizma koje nastaju usled dejstva faktora spoljašnje sredine. Da bi se održala homeostaza i zaštitio organizam, uključuju se brojni adaptacioni mehanizmi. Stresori koji deluju trenutno ili kratkoročno izazivaju akutni stres koji ima protektivni efekat. Kada stresor deluje duže, a organizam ne uspeva da odgovori na izazov, javlja se hronični stres koji vodi u patološko stanje. Hronični stres je, kao etiološki faktor, doveden u vezu sa poremećajima menstrualnog ciklusa žena. Cilj: Cilj je ispitati dejstvo hroničnog imobilizacionog stresa na estrusni ciklus kod ženki miševa NMRI soja. Materijal i metode: Dvanaest polno zrelih ženki miševa NMRI soja nasumično je podeljeno u kontrolnu (n = 6) i eksperimentalnu grupu (n = 6). Eksperimentalnoj grupi je indukovan hronični stres ubacivanjem u imobilizacione komore 2 sata dnevno u periodu od 14 dana. Svakodnevno je svim životinjama uziman vaginalni ispirak od koga je pravljen razmaz radi ispitivanja pod svetlosnim mikroskopom. Analiza preparata je obuhvatala određivanje faza estrusnog ciklusa. Za analizu efekta stresa statistički je ispitana učestalost smenjivanja estrusnih faza u eksperimentalnoj grupi naspram kontrolne. Rezultati: Kod ženki miševa je uočen estrusni ciklus koji se sastoji iz četiri faze: proestrusa, estrusa, metestrusa i diestrusa. U adaptacionom periodu je zapažen prolongirani diestrus, dominantno prisutan u obe grupe. Kod stresiranih jedinki je uočen izlazak iz diestrusa i pojava drugih estrusnih faza, za razliku od kontrolnih kod kojih je diesterus bio znatno učestaliji. Zapažene su neregularnosti u trajanju i smenjivanju faza kod jedinki eksperimentalne grupe, gde se učestalost odstupanja od diestrusa pokazala kao statistički značajna (p < 0,01). Zaključak: Indukovanje imobilizacionog stresa dovodi do izlaska životinja iz diestrusa i velikih varijacija u trajanju i smenjivanju faza estrusnog ciklusa ženki miševa soja NMRI. S obzirom na to da je hormonski mehanizam regulacije estrusnog ciklusa kod ženki miševa soja NMRI sličan mehanizmu regulacije menstrualnog ciklusa kod žena, mogu poslužiti kao dobar model za proučavanje reproduktivnih poremećaja kod žena.},
keywords = {},
pubstate = {published},
tppubtype = {article}
}

@article{Đurić2021,
title = {Direktna imunofluorecencija kao niskobudžetna metoda za ispitivanje moždanog tkiva tokom standardizacije modela neuroborelioze kod miševa soja NMRI},
author = {Selena Đurić and Verica Simin and Pavle Banović},
editor = {Prof. dr Dragutin Arsić},
year = {2021},
date = {2021-05-15},
journal = {PONS - Medicinski časopis},
keywords = {},
pubstate = {forthcoming},
tppubtype = {article}
}

@article{Banović2020,
title = {Humans infested with Ixodes ricinus are exposed to a diverse array of tick-borne pathogens in Serbia},
author = {Pavle Banović, Adrian Alberto Díaz-Sánchez, Clemence Galon, Verica Simin, Dragana Mijatović, Dasiel Obregón, Sara Moutailler, AlejandroCabezas-Cruz
},
url = {https://www.sciencedirect.com/science/article/abs/pii/S1877959X20304799?fbclid=IwAR3nbGgtSfPEXPX54Vr7DTBcHI_KlKPum63yjfSznOTm7NFkh7PyqLzerTs},
doi = {https://doi.org/10.1016/j.ttbdis.2020.101609},
year = {2020},
date = {2020-11-24},
journal = {Ticks and Tick-borne Diseases},
keywords = {},
pubstate = {published},
tppubtype = {article}
}

@article{Verica2020,
title = {Borellia burgdorferi infection in removed ticks and anti-borrelia antibodies in infested patients admitted to the Pasteur Institute, Novi Sad},
author = {Simin Verica and Dragana Mijatović and Snežana Tomanović and Milan Miljević and Borislav Čabrilo and Bogdan Ivana and Pavle Banović},
doi = {10.2298/VETGL200527008S},
year = {2020},
date = {2020-10-03},
journal = {Veterinarski glasnik},
abstract = {Introduction. The primary objectives of this study were (1) to determine the presence of Borrelia burgdorferi infection in ticks removed from patients for the purpose of singling out sites with increased risk of Lyme borreliosis, and (2) to determine the presence of IgM and/or IgG antibodies against B. burgdorferi sensu lato (s. l.) complex in sera of patients who had ticks removed
Materials and Methods. From 108 ticks removed from patients, all were examined zoologically and a sub-sample of 91 ticks was tested using PCR analysis to determine the presence of DNA indicating B. burgdorferi infection. To detect anti-Borrelia IgM and/or IgG antibodies in 61 patients bitten by ticks, we used line recombinant immunoblot test.
Results and Conclusions. The most common tick identified was Ixodes ricinus. B. burgdorferi s. l. was present in 37 of 91 tested ticks (40.7%). Seroconversion against B. burgdorferi s. l. antigen was detected in 12 of 61 patients (19.7%). Most of the infected ticks were from the province of Vojvodina (11 municipalities), with the city of Novi Sad proving to be the site with the highest number of infected ticks, 6 in total. },
keywords = {},
pubstate = {published},
tppubtype = {article}
}

@article{Gordana2020,
title = {Molecular evaluation of chronic restrain stress in mice model of non metastatic fibrosarcoma},
author = {Smieško Gordana and Banović Pavle and Gusman Vera and Simin Verica and Anca Maria Cimpean and Dušan Lalošević},
url = {https://link.springer.com/article/10.1007/s10735-020-09886-5},
doi = {https://doi.org/10.1007/s10735-020-09886-5},
year = {2020},
date = {2020-06-18},
journal = {Journal of Molecular Histology},
abstract = {Chronic stress is regarded as a significant factor in the etiology of the many diseases. Numerous methods have been developed through which the effect of chronic stress is examined. The aim of this study is to demonstrate the new experimental model for analysis of immuno-suppression induced by chronic restraint stress, through challenge with conditionally tumorigenic cell line BHK-21/C13. 20 male NMRI mice were randomly divided into 2 groups—control and experimental. Each mouse was subcutaneously inoculated with BHK-21/C13 cells. Stress in the experimental group was induced for 20 days. After the experiment, tumor masses were removed, and analyzed using histology and immunohistochemistry techniques. We found a statistically significant difference (p = 0.034) in tumor expression and tumor volumes (p = 0.0061) between groups, as well as in immunopositivity on Ki67, cytochrome C and matrix metalloproteinase 9. Absence of immune infiltrate was noticed in experimental, and the presence of inflammatory infiltrate at tumor invasion front in control group.},
keywords = {},
pubstate = {published},
tppubtype = {article}
}