Публикације и научни радови Пастеровог завода…
ИСТРАЖИВАЊА
2024 |
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Banović, Pavle; Mijatović, Dragana; Simin, Verica; Vranješ, Nenad; Meletis, Eleftherios; Kostoulas, Polychronis; Obregon, Dasiel; Cabezas-Cruz, Alejandro Real-world evidence of rabies post-exposure prophylaxis in Serbia: Nation-wide observational study (2017–2019) Journal Article In: Travel Medicine and Infectious Disease, 2024. Садржај | Линкови | BibTeX | Ознаке: immunization, Rabies, seroconversion, vaccine @article{nokey, Background Rabies remains a deadly zoonotic disease, primarily prevalent in Eastern European countries, with a significant global burden in Asia and Africa. Post-exposure prophylaxis (PEP) is critical to prevent clinical rabies. Serbia, a country with a relatively low animal rabies incidence, has been implementing a 4-dose Essen PEP regimen for 13 years. This real-world study aimed to assess the effectiveness of the 4-dose Essen regimen, considering demographic and clinical factors, after WHO Category III exposure. Method The study included 601 patients who received the 4-dose Essen PEP and 79 who received an additional 5th dose. Results Age emerged as a critical factor influencing seroconversion rates after the 4-dose regimen, with older individuals exhibiting lower RVNA titers. Logistic regression indicated a 3.18% decrease in seroconversion odds for each added year of age. The Cox proportional hazards mixed model highlighted age-related risks, with age groups 45–60 and 75–92 at the highest risk of non-seroconversion. Human Rabies Immune Globulin (HRIG) administration was associated with lower RVNA values after the 4-dose regimen, suggesting interference with vaccine immunogenicity among people who received larger doses of HRIG. Conclusions This study provides valuable real-world evidence for rabies PEP in a non-homogeneous population with potential comorbidities. The results underscore the importance of optimizing PEP strategies, particularly in older individuals, and reconsidering HRIG dosing to improve seroconversion rates. | |
2021 |
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Banović, Pavle; Díaz-Sánchez, Adrian Alberto; Mijatović, Dragana; Vujin, Dragana; Zsolt, Horvath; Vranješ, Nenad; Budakov-Obradovic, Zorana; Bujandric, Nevenka; Grujic, Jasmina; Gaffar, Abdul; Jabbar, Abdul; Simin, Verica; Obregon, Dasiel; Cabezas-Cruz, Alejandro Shared Odds of Borrelia and Rabies Virus Exposure in Serbia Journal Article In: Pathogens, vol. 10, no. 4, 2021. Садржај | Линкови | BibTeX | Ознаке: Borrelia, Rabies, risk odds, Serbia @article{Banović2021b, Lyme borreliosis (LB) is the most common tick-borne disease in Serbia and other European countries. Rabies is a fatal zoonosis distributed worldwide and is caused by the rabies virus. Professionals at risk of rabies—including veterinarians, hunters, communal service workers, and forestry workers—overlap with some professions at a higher risk of exposure to tick bites and tick-borne pathogen infections. We hypothesized that individuals identified by the public health system as at risk of rabies virus infection, and consequently vaccinated against rabies virus, also share a higher likelihood of Borrelia exposure. To test our hypothesis, a case-control study was carried out during 2019 in Serbia to determine the seroprevalence of anti-Borrelia antibodies in two case groups (individuals at risk and vaccinated against rabies virus) and a control group (individuals without risk of rabies). Individuals vaccinated against rabies following either “pre-exposure protocol” (PrEP, n = 58) or “post-exposure protocol” (PEP, n = 42) were considered as rabies risk groups and healthy blood donors (n = 30) as the control group. The results showed higher Borrelia seroprevalence in PrEP (17.2%; 10/58) and PEP (19.0%; 8/42) groups compared with the control group (6.67%; 2/30). Furthermore, odds ratio (OR) analysis showed that risk of rabies (in either the PrEP (OR = 2.91) or PEP (OR = 3.29) groups) is associated with increased odds of being seropositive to Borrelia. However, the difference in Borrelia seroprevalence between groups was not statistically significant (Chi-square (χ²) test p > 0.05). The shared odds of LB and rabies exposure found in this study suggest that, in countries where both diseases occur, the common citizen can be at risk of both diseases when in a risky habitat. These findings are important to guide physicians in targeting high-risk groups, and diagnose LB, and to guide decision-makers in targeting control and prevention measures for both infections in risk areas. | |
2016 |
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Lalošević, Dušan; Lalošević, Vesna; Simin, Verica; Miljević, Milan; Čabrilo, Borislav; Čabrilo, Olivera Bjelić Spreading of multilocular echinococcosis in southern Europe: 5 the first record in foxes and jackals in Serbia, Vojvodina Province Journal Article In: European Journal of Wildlife Research, vol. 62, no. 6, pp. 793-796, 2016, ISSN: 1612-4642. Садржај | Линкови | BibTeX | Ознаке: carnivores, echinococcosis, Echinococcus multilocularis Search subject for Echinococcus multilocularis, foxes, intestines, jackals, monitoring, parasites, Rabies, Serbia, Southern European region @article{article, Foxes and jackals from the Vojvodina Province of Serbia were examined for the presence of echinococcosis. Animals were collected as part of routine rabies monitoring and autopsied; their intestines were checked for parasites. Out of 112 examined foxes, echinococcosis was found in 20 (17.9 %); of 28 examined jackals, 4 were infected (14.3 %). Morphological analysis confirmed the presence of Echinococcus multilocularis. This is the first record of E. multilocularis in foxes or other carnivorous mammals in Serbia. | |
2014 |
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AIKIMBAYEV, Alim; BRIGGS, Deborah; COLTAN, Gabriel; Dodet, Betty; Farahtaj, Firouzeh; IMNADZE, Paata; KOREJWO, Joanna; MOISEIEVA, Anna; Usluer, Gaye; Tordo, Noel; Vodopija, Rodovan; Vranješ, Nenad Fighting Rabies in Eastern Europe, the Middle East and Central Asia - Experts Call for a Regional Initiative for Rabies Elimination Journal Article In: Zoonoses and Public Health, pp. 219–226, 2014. Садржај | Линкови | BibTeX | Ознаке: public health programmes, Rabies, rabies control, zoonosis @article{article, MEEREB is an informal network of rabies experts from the Middle East, Eastern Europe and Central Asia, seeking to eliminate rabies from the region. They met for the second time to review the current rabies situation, both globally and in their respective countries, highlighting current rabies control problems and potential solutions. Success stories in Latin America, in Western Europe, in some Asian countries, as well as in Croatia and Serbia prove that elimination of human rabies is achievable in the MEEREB region. It requires political willingness and cooperation of all stakeholders, including Ministries of Health and of Agriculture; adequate management of animal bites through post-exposure prophylaxis; pre-exposure prophylaxis for populations at high risk of rabies exposure, animal vaccination and humane control of stray dog populations. MEEREB members called for a regional initiative for rabies elimination in Eastern Europe and the Middle East. They are confident that the elimination of human rabies of canine origin can be achieved in the region through adopting a One Health approach, and that campaigns for rabies elimination will have significant benefit for public health, including strengthening the structure for control of other zoonoses. | |
2011 |
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McElhinney, L. M.; Marston, D. A.; Freuling, C. M.; Cragg, W.; Stankov, Srđan; Lalošević, Dušan; Müller, T.; Fooks, A. R. Molecular diversity and evolutionary history of rabies virus strains circulating in the Balkans Journal Article In: Journal of General Virology, no. 92, pp. 2171–2180, 2011. Садржај | Линкови | BibTeX | Ознаке: Rabies, rabies virus (RABV), Rabv @article{article, Molecular studies of European classical rabies viruses (RABV) have revealed a number of geographically clustered lineages. To study the diversity of Balkan RABV, partial nucleoprotein (N) gene sequences were analysed from a unique panel of isolates (n5210), collected from various hosts between 1972 and 2006. All of the Balkan isolates grouped within the European/Middle East Lineage, with the majority most closely related to East European strains. A number of RABV from Bosnia & Herzegovina and Montenegro, collected between 1986 and 2006, grouped with the West European strains, believed to be responsible for the rabies epizootic that spread throughout Europe in the latter half of the 20th Century. In contrast, no Serbian RABV belonged to this sublineage. However, a distinct group of Serbian fox RABV provided further evidence for the southwards wildlife-mediated movement of rabies from Hungary, Romania and Serbia into Bulgaria. To determine the optimal region for evolutionary analysis, partial, full and concatenated N-gene and glycoprotein (G) gene sequences were compared. Whilst both the divergence times and evolutionary rates were similar irrespective of genomic region, the 95% highest probability density (HPD) limits were significantly reduced for full N-gene and concatenated NG-gene sequences compared with partial gene sequences. Bayesian coalescent analysis estimated the date of the most common recent ancestor of the Balkan RABV to be 1885 (95% HPD, 1852–1913), and skyline plots suggested an expansion of the local viral population in 1980–1990, which coincides with the observed emergence of fox rabies in the region. | |
2010 |
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Aylan, Orhan; El-Sayed, Aly Fahmy Mohamed; Farahtaj, Firouzeh; Janani, Ali R.; Lugach, Olga; Tarkhan-Mouravi, Olgha; Usluer, Gaye; Vodopija, Rodovan; Vranješ, Nenad; Tordo, Noel; Dodet, Betty Report of the First Meeting of the Middle East and Eastern Europe Rabies Expert Bureau, Istanbul, Turkey Journal Article In: SAGE-Hindawi Access to Research, Advances in Preventive Medicine, vol. 2011, no. 812515, pp. 4, 2010. Садржај | Линкови | BibTeX | Ознаке: Rabies @article{article812515, Rabies is a threat in all parts of the world where animal reservoirs persists, including Eastern Europe and the Middle East. Rabies experts from seven Middle East and Eastern European countries (Croatia, Egypt, Georgia, Iran, Serbia, Turkey, and Ukraine) met for two days in Istanbul, Turkey (June 8-9, 2010), to exchange information on the epidemiological situation concerning human and animal rabies in their respective countries and to discuss strategies for rabies elimination and control. They decided to establish a regional network, the Middle East and Eastern Europe Rabies Expert Bureau (MEEREB), a regional network of experts, to increase collaboration in rabies prevention and control at the local, regional, and global levels. | |
2008 |
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Lalošević, Dušan; Lalošević, Vesna; Lazarević-Ivanc, Lj; Knežević, Ivana BHK-21 cell culture rabies vaccine: Lmmunogenicity of a candidate vaccine for humans Journal Article In: Developments in biologicals, vol. 131, pp. 421-9, 2008. Садржај | Линкови | BibTeX | Ознаке: BHK-21/C13, Rabies @article{article, Veterinary rabies vaccines produced in BHK-21/C13 permanent cell cultures have been used for a long period of time and have been proven as efficacious and safe. A candidate vaccine for human use (YU BHK Rabivak) was developed at the Pasteur Institute, Novi Sad, Serbia on the basis of the fixed rabies virus strain "L. Pasteur 2061/Vero 15 pas" using BHK 21/C13 as a cell substrate for vaccine production. To test the vaccine immunogenicity, a clinical trial was conducted involving 164 subjects between 18 and 60 years of age, immunized either with the YU BHK Rabivak vaccine candidate orwith a commercially available vaccine (Rabipur). Three groups of subjects were immunized with either vaccine by intramuscular administration in the deltoid region, following a pre-exposure regimen on days 0, 7 and 21, or the Essen or Zagreb post-exposure regimens. Rabies virus neutralizing antibodies (VNA) titres were determined by rapid fluorescent focus inhibition test (RFFIT) 21 and 30-45 days post vaccination. A protective titre of VNAs (>0.5 IU/ml) was found in all subjects vaccinated. Dynamics of the immune response showed that 96.4% of the subjects developed protective VNA titres after two doses, 99.3% after three doses and 100% after four and five doses of the candidate YU BHK Rabivak vaccine. There was a low reactogenicity without serious adverse events indicating a satisfactory safety profile in humans. Results obtained in this study indicate that BHK 21 cells offer the possibility of producing an efficacious and safe cell-culture rabies vaccine for humane use. |