Публикације и научни радови Пастеровог завода…
ИСТРАЖИВАЊА
2020 |
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Verica, Simin; Mijatović, Dragana; Tomanović, Snežana; Miljević, Milan; Čabrilo, Borislav; Ivana, Bogdan; Banović, Pavle Borellia burgdorferi infection in removed ticks and anti-borrelia antibodies in infested patients admitted to the Pasteur Institute, Novi Sad Journal Article In: Veterinarski glasnik, 2020. Садржај | Линкови | BibTeX | Ознаке: Borrelia, Borrelia burgdorferi, Lyme borreliosis, Novi Sad, ticks @article{Verica2020, Introduction. The primary objectives of this study were (1) to determine the presence of Borrelia burgdorferi infection in ticks removed from patients for the purpose of singling out sites with increased risk of Lyme borreliosis, and (2) to determine the presence of IgM and/or IgG antibodies against B. burgdorferi sensu lato (s. l.) complex in sera of patients who had ticks removed Materials and Methods. From 108 ticks removed from patients, all were examined zoologically and a sub-sample of 91 ticks was tested using PCR analysis to determine the presence of DNA indicating B. burgdorferi infection. To detect anti-Borrelia IgM and/or IgG antibodies in 61 patients bitten by ticks, we used line recombinant immunoblot test. Results and Conclusions. The most common tick identified was Ixodes ricinus. B. burgdorferi s. l. was present in 37 of 91 tested ticks (40.7%). Seroconversion against B. burgdorferi s. l. antigen was detected in 12 of 61 patients (19.7%). Most of the infected ticks were from the province of Vojvodina (11 municipalities), with the city of Novi Sad proving to be the site with the highest number of infected ticks, 6 in total. | |
2017 |
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Ristanović, Elizabeta; Atanasievska, Sonja; Protić-Đokić, Vesna; Hinić, Nataša; Lalošević, Dušan MICROBIOLOGICAL DETECTION OF BORRELIA BURGDORFERI AND CONTEMPORARY DIAGNOSIS OF LYME BORELLOSIS Journal Article In: MD Medical review, vol. 9, no. 3, pp. 143-147, 2017. Садржај | Линкови | BibTeX | Ознаке: Borrelia burgdorferi, Lyme borreliosis, PCR method, Serological tests @article{article, Lyme borreliosis is a multi-system disease caused by Borrelia burgdorferi, which is transmitted by hard ticks Ixodes spp. The most common clinical manifestation is the skin lesion (erythema migrans), however there is a tendency of the pathogen to spread and cause damages to the joints, nervous and cardiovascular system. The diagnosis of Lyme disease is based primarily on clinical findings, epidemiological and anamnestic data and laboratory test results. The application of the modern real-time PCR method allows us to test the skin in tick bite area for a presence of B. burgdorferi. In the diagnosis of Lyme disease, we often use indirect methods (TIIF, ELISA, Western blot) for detecting specific IgM and IgG antibodies against B. burgdorferi in serum, liquor and synovial fluid. To confirm the diagnosis, the presence of IgG antibodies should be demonstrated, since the presence of IgM is not a relible indicator of a recent infection. When interpreting immunodiagnostic test results it has to be considered that there are a lot of variability as the phenotype characteristics of borrelia, different antigenic structures, different geographical distribution, phase varies in duration of the disease, characteristics of individual immune responses, presence of other diseases as well as the applied antibiotic treatment, which all can affect the outcome. Due to the high presence of infected ticks in our geographical area, it is necessary to provide continuous preventive measures, epidemiological monitoring and improvement of the diagnostic and therapeutic approach in the treatment of Lyme borreliosis. |