Publications and scientific papers of Pasteur Institute…
Verica, Simin; Mijatović, Dragana; Tomanović, Snežana; Miljević, Milan; Čabrilo, Borislav; Ivana, Bogdan; Banović, Pavle
In: Veterinarski glasnik, 2020.
Introduction. The primary objectives of this study were (1) to determine the presence of Borrelia burgdorferi infection in ticks removed from patients for the purpose of singling out sites with increased risk of Lyme borreliosis, and (2) to determine the presence of IgM and/or IgG antibodies against B. burgdorferi sensu lato (s. l.) complex in sera of patients who had ticks removed
Materials and Methods. From 108 ticks removed from patients, all were examined zoologically and a sub-sample of 91 ticks was tested using PCR analysis to determine the presence of DNA indicating B. burgdorferi infection. To detect anti-Borrelia IgM and/or IgG antibodies in 61 patients bitten by ticks, we used line recombinant immunoblot test.
Results and Conclusions. The most common tick identified was Ixodes ricinus. B. burgdorferi s. l. was present in 37 of 91 tested ticks (40.7%). Seroconversion against B. burgdorferi s. l. antigen was detected in 12 of 61 patients (19.7%). Most of the infected ticks were from the province of Vojvodina (11 municipalities), with the city of Novi Sad proving to be the site with the highest number of infected ticks, 6 in total.
Pavle, Banović; Čapo, Ivan; Ogorelica, Dejan; Vranješ, Nenad; Verica, Simin; Lalošević, Dušan
In: Future Microbiology, 2020.
The majority of suggested mechanisms of Borrelia spreading inside erythema migrans (EM) are developed from in vitro studies and animal models. This report is the first to describe pathomorphological substrate of EM caused by Borrelia spielmanii in humans, addressing the hypothesis of enhanced Borrelia penetration through extracellular matrix. In the process of ruling out of atypical Masters’ disease, we conducted a punch biopsy of suspected EM and a two-tier serology testing for Lyme borreliosis, where we registered antibodies against B. spielmanii. Skin biopsy showed CD4+ and CD8+ lymphocyte involvement and high activity of matrix metalloproteinase 9. No alterations were detected in distribution and morphology of collagen type I and IV. Therefore, it is suggested that other mechanisms should be considered as major contributing factors to local spreading of B.spielmanii.
Ristanović, Elizabeta; Atanasievska, Sonja; Protić-Đokić, Vesna; Hinić, Nataša; Lalošević, Dušan
In: MD Medical review, 9 (3), pp. 143-147, 2017.
Lyme borreliosis is a multi-system disease caused by Borrelia burgdorferi, which is transmitted by hard ticks Ixodes spp. The most common clinical manifestation is the skin lesion (erythema migrans), however there is a tendency of the pathogen to spread and cause damages to the joints, nervous and cardiovascular system. The diagnosis of Lyme disease is based primarily on clinical findings, epidemiological and anamnestic data and laboratory test results. The application of the modern real-time PCR method allows us to test the skin in tick bite area for a presence of B. burgdorferi. In the diagnosis of Lyme disease, we often use indirect methods (TIIF, ELISA, Western blot) for detecting specific IgM and IgG antibodies against B. burgdorferi in serum, liquor and synovial fluid. To confirm the diagnosis, the presence of IgG antibodies should be demonstrated, since the presence of IgM is not a relible indicator of a recent infection. When interpreting immunodiagnostic test results it has to be considered that there are a lot of variability as the phenotype characteristics of borrelia, different antigenic structures, different geographical distribution, phase varies in duration of the disease, characteristics of individual immune responses, presence of other diseases as well as the applied antibiotic treatment, which all can affect the outcome. Due to the high presence of infected ticks in our geographical area, it is necessary to provide continuous preventive measures, epidemiological monitoring and improvement of the diagnostic and therapeutic approach in the treatment of Lyme borreliosis.
Lalošević, Dušan; Lalošević, Vesna; Stojšić-Milosavljević, Anastazija; Stojšić, Đurica
In: International Journal of Cardiology, 145 (3), pp. e96–e98, 2010.
A case of a patient who developed an acute myocarditis due to Lyme disease is reported. An increased serum antibody titer to Borrelia burgdorferi suggested a diagnosis and in addition of basic clinical methods, endomyocardial biopsy performed and analyzed by transmission electron microscopy. The lumen of myocardial capillaries was founded mostly filled with detritus and fibrin precipitate, between them several bacterial fragments were identified. The electron-microscopic characteristics of the microorganisms in this specimen, revealing irregularly coiled appearance and consistent thickness of 0.2 μm, correspond to the spiral-like structure of Lyme disease borrelia. The presence of fibrin deposits on the capillary endothelium and necrosis of myocardiocytes, suggests that the cardiopathy in our patient was represent borrelia-mediated damage of the hearth microcirculation.