Publications and scientific papers of Pasteur Institute…
PUBLICATIONS
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2020 |
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Gordana, Smieško; Pavle, Banović; Vera, Gusman; Verica, Simin; Cimpean, Anca Maria; Lalošević, Dušan Molecular evaluation of chronic restrain stress in mice model of non metastatic fibrosarcoma Journal Article Journal of Molecular Histology, 2020. Abstract | Links | BibTeX | Tags: @article{Gordana2020, title = {Molecular evaluation of chronic restrain stress in mice model of non metastatic fibrosarcoma}, author = {Smieško Gordana and Banović Pavle and Gusman Vera and Simin Verica and Anca Maria Cimpean and Dušan Lalošević}, url = {https://link.springer.com/article/10.1007/s10735-020-09886-5}, doi = {https://doi.org/10.1007/s10735-020-09886-5}, year = {2020}, date = {2020-06-18}, journal = {Journal of Molecular Histology}, abstract = {Chronic stress is regarded as a significant factor in the etiology of the many diseases. Numerous methods have been developed through which the effect of chronic stress is examined. The aim of this study is to demonstrate the new experimental model for analysis of immuno-suppression induced by chronic restraint stress, through challenge with conditionally tumorigenic cell line BHK-21/C13. 20 male NMRI mice were randomly divided into 2 groups—control and experimental. Each mouse was subcutaneously inoculated with BHK-21/C13 cells. Stress in the experimental group was induced for 20 days. After the experiment, tumor masses were removed, and analyzed using histology and immunohistochemistry techniques. We found a statistically significant difference (p = 0.034) in tumor expression and tumor volumes (p = 0.0061) between groups, as well as in immunopositivity on Ki67, cytochrome C and matrix metalloproteinase 9. Absence of immune infiltrate was noticed in experimental, and the presence of inflammatory infiltrate at tumor invasion front in control group.}, keywords = {}, pubstate = {published}, tppubtype = {article} } Chronic stress is regarded as a significant factor in the etiology of the many diseases. Numerous methods have been developed through which the effect of chronic stress is examined. The aim of this study is to demonstrate the new experimental model for analysis of immuno-suppression induced by chronic restraint stress, through challenge with conditionally tumorigenic cell line BHK-21/C13. 20 male NMRI mice were randomly divided into 2 groups—control and experimental. Each mouse was subcutaneously inoculated with BHK-21/C13 cells. Stress in the experimental group was induced for 20 days. After the experiment, tumor masses were removed, and analyzed using histology and immunohistochemistry techniques. We found a statistically significant difference (p = 0.034) in tumor expression and tumor volumes (p = 0.0061) between groups, as well as in immunopositivity on Ki67, cytochrome C and matrix metalloproteinase 9. Absence of immune infiltrate was noticed in experimental, and the presence of inflammatory infiltrate at tumor invasion front in control group. | |
Fedrigolli, Elsa; Bogdan, Damir; Lalošević, Dušan; Banović, Pavle Proposition Of A Simplified Protocol and New Parameter Introduction In NMRI Mice Anhedonia Induction Journal Article Forthcoming Serbian Journal of Experimental and Clinical Research, In press , Forthcoming. BibTeX | Tags: anhedonia, anxiety, Chronic stress protocol, depression, NMRI mice @article{Fedrigolli2020, title = {Proposition Of A Simplified Protocol and New Parameter Introduction In NMRI Mice Anhedonia Induction}, author = {Elsa Fedrigolli and Damir Bogdan and Dušan Lalošević and Pavle Banović}, year = {2020}, date = {2020-04-30}, journal = {Serbian Journal of Experimental and Clinical Research}, volume = {In press}, keywords = {anhedonia, anxiety, Chronic stress protocol, depression, NMRI mice}, pubstate = {forthcoming}, tppubtype = {article} } | |
Stankov, Srđan; Obradović, Nemanja; Vujin, Dragana; Vranješ, Nenad Possibility of Immediate Introduction of a Single-Dose Antibody Induction Test as a Refinement of the NIH Test for Inactivated Rabies Vaccine Potency Determination Journal Article Forthcoming Viral Immunology, pp. 1-11, Forthcoming. Abstract | Links | BibTeX | Tags: animal welfar, antibody induction test, inactivated rabies vaccine, NIH test, potency determination @article{Stankov2020, title = {Possibility of Immediate Introduction of a Single-Dose Antibody Induction Test as a Refinement of the NIH Test for Inactivated Rabies Vaccine Potency Determination}, author = {Srđan Stankov and Nemanja Obradović and Dragana Vujin and Nenad Vranješ}, url = {https://www.liebertpub.com/doi/10.1089/vim.2019.0131}, doi = {10.1089/vim.2019.0131}, year = {2020}, date = {2020-03-10}, journal = {Viral Immunology}, pages = {1-11}, abstract = {Antibody induction test (AIT) is a promising candidate as a refinement of the troublesome National institutes of Health (NIH) test in the sense of animal welfare 3R approach for determination of potency of inactivated rabies vaccines for veterinary and human use. In this study, we initially try to develop AIT as a suitable alternative to NIH test, to achieve a reduction of test duration and diminish animal suffering by omitting intracerebral CVS infection and measuring humoral immunity upon vaccination. Designs of both multi-dose and single-dose AIT were examined. Biological reference preparation, batch 5 with assigned titer of 10 IU/vial, was taken as both standard and test vaccine. Six consecutive AITs were performed and eight pools of sera in each AIT were tested in triplicate by rapid fluorescent focus inhibition test. We estimated the upper detection limit and calculated test variability for individual dilutions. For multi-dose AIT, we estimated the dose–response function and performed calculations of final test results and statistical validity parameters for both linear and sigmoidal model using CombiStats program. Sigmoidal 4-parameter dose–response model was found optimal. Presented design of multi-dose AIT showed a satisfactory detection limit for testing of inactivated rabies vaccines for both veterinary and human use. However, due to nonconformity of obtained results with statistical validity criteria, we concluded that the presented model of multi-dose AIT was unsuitable for introduction in routine practice. However, we concluded that there was a realistic option for introduction of two versions of single-dose AIT. The first version would be with two standard vaccine controls and could be introduced immediately, while the second version would include testing of the sample only and rely on comparison of the induced rabies antibody level with absolute cut-off limits set in advance.}, keywords = {animal welfar, antibody induction test, inactivated rabies vaccine, NIH test, potency determination}, pubstate = {forthcoming}, tppubtype = {article} } Antibody induction test (AIT) is a promising candidate as a refinement of the troublesome National institutes of Health (NIH) test in the sense of animal welfare 3R approach for determination of potency of inactivated rabies vaccines for veterinary and human use. In this study, we initially try to develop AIT as a suitable alternative to NIH test, to achieve a reduction of test duration and diminish animal suffering by omitting intracerebral CVS infection and measuring humoral immunity upon vaccination. Designs of both multi-dose and single-dose AIT were examined. Biological reference preparation, batch 5 with assigned titer of 10 IU/vial, was taken as both standard and test vaccine. Six consecutive AITs were performed and eight pools of sera in each AIT were tested in triplicate by rapid fluorescent focus inhibition test. We estimated the upper detection limit and calculated test variability for individual dilutions. For multi-dose AIT, we estimated the dose–response function and performed calculations of final test results and statistical validity parameters for both linear and sigmoidal model using CombiStats program. Sigmoidal 4-parameter dose–response model was found optimal. Presented design of multi-dose AIT showed a satisfactory detection limit for testing of inactivated rabies vaccines for both veterinary and human use. However, due to nonconformity of obtained results with statistical validity criteria, we concluded that the presented model of multi-dose AIT was unsuitable for introduction in routine practice. However, we concluded that there was a realistic option for introduction of two versions of single-dose AIT. The first version would be with two standard vaccine controls and could be introduced immediately, while the second version would include testing of the sample only and rely on comparison of the induced rabies antibody level with absolute cut-off limits set in advance. | |
 | Pavle, Banović; Čapo, Ivan; Ogorelica, Dejan; Vranješ, Nenad; Verica, Simin; Lalošević, Dušan Mysterious path of Borrelia spielmanii: spreading without morphological alteration of collagen type I and IV Journal Article Future Microbiology, 2020. Abstract | Links | BibTeX | Tags: B. spielmanii, Borrelia, collagen type I, collagen type IV, erythema migrans, Lyme borreliosis, MMP9 @article{Pavle2020, title = {Mysterious path of Borrelia spielmanii: spreading without morphological alteration of collagen type I and IV}, author = {Banović Pavle and Ivan Čapo and Dejan Ogorelica and Nenad Vranješ and Simin Verica and Dušan Lalošević}, url = {https://www.futuremedicine.com/doi/10.2217/fmb-2019-0217}, doi = {https://doi.org/10.2217/fmb-2019-0217}, year = {2020}, date = {2020-01-06}, journal = {Future Microbiology}, abstract = {The majority of suggested mechanisms of Borrelia spreading inside erythema migrans (EM) are developed from in vitro studies and animal models. This report is the first to describe pathomorphological substrate of EM caused by Borrelia spielmanii in humans, addressing the hypothesis of enhanced Borrelia penetration through extracellular matrix. In the process of ruling out of atypical Masters’ disease, we conducted a punch biopsy of suspected EM and a two-tier serology testing for Lyme borreliosis, where we registered antibodies against B. spielmanii. Skin biopsy showed CD4+ and CD8+ lymphocyte involvement and high activity of matrix metalloproteinase 9. No alterations were detected in distribution and morphology of collagen type I and IV. Therefore, it is suggested that other mechanisms should be considered as major contributing factors to local spreading of B.spielmanii.}, keywords = {B. spielmanii, Borrelia, collagen type I, collagen type IV, erythema migrans, Lyme borreliosis, MMP9}, pubstate = {published}, tppubtype = {article} } The majority of suggested mechanisms of Borrelia spreading inside erythema migrans (EM) are developed from in vitro studies and animal models. This report is the first to describe pathomorphological substrate of EM caused by Borrelia spielmanii in humans, addressing the hypothesis of enhanced Borrelia penetration through extracellular matrix. In the process of ruling out of atypical Masters’ disease, we conducted a punch biopsy of suspected EM and a two-tier serology testing for Lyme borreliosis, where we registered antibodies against B. spielmanii. Skin biopsy showed CD4+ and CD8+ lymphocyte involvement and high activity of matrix metalloproteinase 9. No alterations were detected in distribution and morphology of collagen type I and IV. Therefore, it is suggested that other mechanisms should be considered as major contributing factors to local spreading of B.spielmanii. |
2019 |
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| Miljević, Milan; Čabrilo, Olivera Bjelić; Simin, Verica; Čabrilo, Borislav; Miljević, Jelena Boganč; Lalošević, Dušan Significance of the red fox as a natural reservoir of intestinal zoonoses in Vojvodina, Serbia Journal Article Acta Veterinaria Hungarica, 64 (4), 2019, ISSN: 1588-2705. Abstract | Links | BibTeX | Tags: @article{Miljević2019, title = {Significance of the red fox as a natural reservoir of intestinal zoonoses in Vojvodina, Serbia}, author = {Milan Miljević and Olivera Bjelić Čabrilo and Verica Simin and Borislav Čabrilo and Jelena Boganč Miljević and Dušan Lalošević}, doi = {https://doi.org/10.1556/004.2019.055}, issn = {1588-2705}, year = {2019}, date = {2019-12-19}, journal = {Acta Veterinaria Hungarica}, volume = {64}, number = {4}, abstract = {In the present study, 223 foxes were collected from various localities in the northern part of the Republic of Serbia (Vojvodina province) and examined for intestinal helminths. Among the examined foxes 178 (79.8%) were infected. The most frequently identified parasites were Mesocestoides spp. (49.3%) and Toxascaris leonina (36.3%). The parasite with the lowest prevalence was Pterygodermatites affinis (0.9%), and this is the first confirmed finding in Serbia. The other recovered species were Alaria alata (25.6%), Taenia spp. (6.3%), Echinococcus multilocularis (13%), Toxocara canis (16.6%) and Uncinaria stenocephala (14.8%). The highest number of foxes infected with E. multilocularis were in the Srem area. The results of this study indicate the presence of helminth species in red foxes in Vojvodina which may also infect humans.}, keywords = {}, pubstate = {published}, tppubtype = {article} } In the present study, 223 foxes were collected from various localities in the northern part of the Republic of Serbia (Vojvodina province) and examined for intestinal helminths. Among the examined foxes 178 (79.8%) were infected. The most frequently identified parasites were Mesocestoides spp. (49.3%) and Toxascaris leonina (36.3%). The parasite with the lowest prevalence was Pterygodermatites affinis (0.9%), and this is the first confirmed finding in Serbia. The other recovered species were Alaria alata (25.6%), Taenia spp. (6.3%), Echinococcus multilocularis (13%), Toxocara canis (16.6%) and Uncinaria stenocephala (14.8%). The highest number of foxes infected with E. multilocularis were in the Srem area. The results of this study indicate the presence of helminth species in red foxes in Vojvodina which may also infect humans. |
Banović, Pavle; Mijatović, Dragana; Ogorelica, Dejan; Vranješ, Nenad; Lalošević, Dušan Aktuelni diferencijalno dijagnosticki problemi i patofiziološki mehanizmi razvoja migratornog eritema kod lajm borelioze Journal Article Medical Data, 11 (3-4), pp. 141-146, 2019. Abstract | Links | BibTeX | Tags: borelija, erythema migrans, lajm borelioza, migratorni eritem @article{Banović2019, title = {Aktuelni diferencijalno dijagnosticki problemi i patofiziološki mehanizmi razvoja migratornog eritema kod lajm borelioze}, author = {Pavle Banović and Dragana Mijatović and Dejan Ogorelica and Nenad Vranješ and Dušan Lalošević}, url = {http://www.md-medicaldata.com/godina_2019_broj_3_title_05.html}, year = {2019}, date = {2019-12-18}, journal = {Medical Data}, volume = {11}, number = {3-4}, pages = {141-146}, abstract = {Migratorni eritem predstavlja najčešću manifestaciju prve (rane) faze lajm borelioze. Definisan je kao crvenilo na mestu uboda krpelja koje se širi. Iako pojava migratornog eritema upućuje na postojanje lokalne infekcije patogenim sojevima bakterija (spiroheta) iz Borrelia burgdorferi sensu lato kompleksa, tačan mehanizam kojim ove spirohete ostvaruju širenje u koži čoveka nisu razjašnjeni. U ovom radu je dat pregled literature, na početku vezan za interakciju na nivou krpelj-patogen-domaćin, nakon čega su predstavljene najčešće teorije razvoja specifične morfologije migratornog eritema, kao i diferencijalno dijagnostički problemi koji mogu nastati usled infekcije drugim patogenima ili usled razvoja različitih alergijskih i autoimunih stanja.}, keywords = {borelija, erythema migrans, lajm borelioza, migratorni eritem}, pubstate = {published}, tppubtype = {article} } Migratorni eritem predstavlja najčešću manifestaciju prve (rane) faze lajm borelioze. Definisan je kao crvenilo na mestu uboda krpelja koje se širi. Iako pojava migratornog eritema upućuje na postojanje lokalne infekcije patogenim sojevima bakterija (spiroheta) iz Borrelia burgdorferi sensu lato kompleksa, tačan mehanizam kojim ove spirohete ostvaruju širenje u koži čoveka nisu razjašnjeni. U ovom radu je dat pregled literature, na početku vezan za interakciju na nivou krpelj-patogen-domaćin, nakon čega su predstavljene najčešće teorije razvoja specifične morfologije migratornog eritema, kao i diferencijalno dijagnostički problemi koji mogu nastati usled infekcije drugim patogenima ili usled razvoja različitih alergijskih i autoimunih stanja. | |
| Glavaški, Mila; Banović, Pavle; Lalošević, Dušan Number and Distribution of Mast Cells in Reproductive Systems of Gravid and Non-Gravid Female Mice Journal Article Forthcoming Serbian Journal of Experimental and Clinical Research, 20 (4), Forthcoming. Abstract | Links | BibTeX | Tags: mast cells @article{Glavaški2019, title = {Number and Distribution of Mast Cells in Reproductive Systems of Gravid and Non-Gravid Female Mice}, author = {Mila Glavaški and Pavle Banović and Dušan Lalošević}, doi = {10.2478/sjecr-2019-0047}, year = {2019}, date = {2019-11-22}, journal = {Serbian Journal of Experimental and Clinical Research}, volume = {20}, number = {4}, abstract = {Mast cells are mainly distributed in tissues exposed to the external environment, but they are also found in ovaries, oviducts and uterus. We determined the difference in the number and distribution of Alcian Blue (+) and Safranine O (+) mast cells in reproductive systems of gravid and non-gravid female mice by histological examination. We showed that the number of mast cells is significantly higher in the reproductive system of gravid mice than in the reproductive system of non-gravid mice. Mast cells are unevenly distributed in ovaries, oviducts and uterusin both gravid and non-gravid mice. A large number of mast cells reside close to blood vessels throughout the entire reproductive system.}, keywords = {mast cells}, pubstate = {forthcoming}, tppubtype = {article} } Mast cells are mainly distributed in tissues exposed to the external environment, but they are also found in ovaries, oviducts and uterus. We determined the difference in the number and distribution of Alcian Blue (+) and Safranine O (+) mast cells in reproductive systems of gravid and non-gravid female mice by histological examination. We showed that the number of mast cells is significantly higher in the reproductive system of gravid mice than in the reproductive system of non-gravid mice. Mast cells are unevenly distributed in ovaries, oviducts and uterusin both gravid and non-gravid mice. A large number of mast cells reside close to blood vessels throughout the entire reproductive system. |
| Gordana, Smieško; Vera, Gusman; Pavle, Banović Jack of all trades spotted in the Clinical Centre of Vojvodina – first detection of Aeromonas hydrophila from urinary tract infection samples Journal Article Srpski arhiv za celokupno lekarstvo, 2019. Abstract | Links | BibTeX | Tags: @article{Gordana2019, title = {Jack of all trades spotted in the Clinical Centre of Vojvodina – first detection of Aeromonas hydrophila from urinary tract infection samples}, author = {Smieško Gordana and Gusman Vera and Banović Pavle}, url = {http://www.srpskiarhiv.rs/global/pdf/onlinefirst/110-19OlF-v1.pdf}, doi = {10.2298/SARH190717110S}, year = {2019}, date = {2019-10-09}, journal = {Srpski arhiv za celokupno lekarstvo}, abstract = {Aeromonas hydrophila is representative of group within the Aeromonadaceae family that mainly cause infections in humans. Aeromonads can induce meningitis, septicemia, respiratory and hemolytic uremic syndrome as well as gastroenteritis. Regarding diverse mechanisms involved in virulence and metabolic adaptation of A. hydrophila to various hosts and environments it is often introduced as “Jack of all trades”. Here we report first isolation of Aeromonas hydrophila from urine samples of the three patients from Urology Department within Clinical Centre of Vojvodina. Epidemiological survey identified contact with surface water as only mutual risk parameter. Following that, novel antibacterial agents against A. hydrophila are discussed.}, keywords = {}, pubstate = {published}, tppubtype = {article} } Aeromonas hydrophila is representative of group within the Aeromonadaceae family that mainly cause infections in humans. Aeromonads can induce meningitis, septicemia, respiratory and hemolytic uremic syndrome as well as gastroenteritis. Regarding diverse mechanisms involved in virulence and metabolic adaptation of A. hydrophila to various hosts and environments it is often introduced as “Jack of all trades”. Here we report first isolation of Aeromonas hydrophila from urine samples of the three patients from Urology Department within Clinical Centre of Vojvodina. Epidemiological survey identified contact with surface water as only mutual risk parameter. Following that, novel antibacterial agents against A. hydrophila are discussed. |
| Lalošević, Dušan; Banović, Pavle; Cvjetković, Boris; Stumpf, Iris; Lalošević, Vesna; Vranješ, Nenad ULCERATED NONHEALING SUBCUTANEOUS NODULE AT THE CAT – A CASE OF MYCETOMA AND FAMILIAL DERMATOMYCOSIS OF OWNERS Journal Article Medical Data, 11 (2), pp. 107-109, 2019. Abstract | BibTeX | Tags: cat, Dermatomycoses, mycetoma, pathohistology @article{Lalošević2019, title = {ULCERATED NONHEALING SUBCUTANEOUS NODULE AT THE CAT – A CASE OF MYCETOMA AND FAMILIAL DERMATOMYCOSIS OF OWNERS}, author = {Dušan Lalošević and Pavle Banović and Boris Cvjetković and Iris Stumpf and Vesna Lalošević and Nenad Vranješ}, year = {2019}, date = {2019-09-20}, journal = {Medical Data}, volume = {11}, number = {2}, pages = {107-109}, abstract = {Micetom kao pseudotumorski infiltrat izazvan gljivama ili nekim bakterijama, retko se vidi u biopsijskom materijalu. Prikazujemo slučaj mačke, koji je povezan i sa gljivičnom infekcijom više članova porodice vlasnika. Vlasnička porodica ima pet članova od kojih su četiri imali dermatomikozu. Mogući uzročnici su Microsporum canis i Coccidioides spp.}, keywords = {cat, Dermatomycoses, mycetoma, pathohistology}, pubstate = {published}, tppubtype = {article} } Micetom kao pseudotumorski infiltrat izazvan gljivama ili nekim bakterijama, retko se vidi u biopsijskom materijalu. Prikazujemo slučaj mačke, koji je povezan i sa gljivičnom infekcijom više članova porodice vlasnika. Vlasnička porodica ima pet članova od kojih su četiri imali dermatomikozu. Mogući uzročnici su Microsporum canis i Coccidioides spp. |
2018 |
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| Mijatović, Dragana; Simin, Verica; Vujin, Dragana; Miljević, Milan; Nikolić, Nataša; Vranješ, Nenad BED BUGS CIMEX LECTULARIUS (LINNAEUS, 1758), NOVI SAD, 2018 – Case report Journal Article MD-Medical Data, 10 (4), pp. 209-2011, 2018. Abstract | Links | BibTeX | Tags: bed bugs, Cimex lectularius, itching @article{articlej, title = {BED BUGS CIMEX LECTULARIUS (LINNAEUS, 1758), NOVI SAD, 2018 – Case report}, author = {Dragana Mijatović and Verica Simin and Dragana Vujin and Milan Miljević and Nataša Nikolić and Nenad Vranješ}, url = {https://www.pasterovzavod.rs/wp-content/uploads/2019/02/08-MD-Vol-10-No-4-Dec-2018_Mijatovic-et-al.pdf}, year = {2018}, date = {2018-12-28}, journal = {MD-Medical Data}, volume = {10}, number = {4}, pages = {209-2011}, abstract = {Bed bugs are ectoparasites of people, chickens, bats and occasionally the domestic animals and feed by the host’s blood. They are nocturnal and come out of their shelter in bed or other furniture. In humans, the place of the bite shows the local reaction that is followed by itching and forming of the crust. 68-years-old female patient reports at the Pasteur Institute for emerging bite-like wounds on the skin followed by strong itching. Physical examination of patient revealed that the causative agent could be insect. After determination of the insects delivered by the patient next day, organism was identified as Cimex lectularius, Linaeus, 1758 - bed bug. The treatment of the patient who are bitten by the bed bugs consist of lowering of itching by local corticosteroids and oral antihistamines and the prevention of the secondary bacterial infection.}, keywords = {bed bugs, Cimex lectularius, itching}, pubstate = {published}, tppubtype = {article} } Bed bugs are ectoparasites of people, chickens, bats and occasionally the domestic animals and feed by the host’s blood. They are nocturnal and come out of their shelter in bed or other furniture. In humans, the place of the bite shows the local reaction that is followed by itching and forming of the crust. 68-years-old female patient reports at the Pasteur Institute for emerging bite-like wounds on the skin followed by strong itching. Physical examination of patient revealed that the causative agent could be insect. After determination of the insects delivered by the patient next day, organism was identified as Cimex lectularius, Linaeus, 1758 - bed bug. The treatment of the patient who are bitten by the bed bugs consist of lowering of itching by local corticosteroids and oral antihistamines and the prevention of the secondary bacterial infection. |
| Lalošević, Dušan; Pobor, Marta; Pete, Maria; Vukobratov, Zita; Banović, Pavle; Ružić, Maja EOSINOPHILIA, DIARRHEA AND MASS OF POLLEN GRAINS AND CHARCOT–LEYDEN CRYSTALS IN THE STOOL – Case report Journal Article MD-Medical Data, 10 (4), pp. 205-207, 2018. Abstract | Links | BibTeX | Tags: diarrhea, Pollen, pollen grains, sensitization @article{article, title = {EOSINOPHILIA, DIARRHEA AND MASS OF POLLEN GRAINS AND CHARCOT–LEYDEN CRYSTALS IN THE STOOL – Case report}, author = {Dušan Lalošević and Marta Pobor and Maria Pete and Zita Vukobratov and Pavle Banović and Maja Ružić}, url = {https://www.pasterovzavod.rs/wp-content/uploads/2019/02/07-MD-Vol-10-No-4-Dec-2018_Lalosevic-et-al.pdf}, year = {2018}, date = {2018-12-25}, journal = {MD-Medical Data}, volume = {10}, number = {4}, pages = {205-207}, abstract = {A 62-year-old patient, in good condition, reported to the doctor for vomiting and diarrhea. A month after the beginning of oral bee pollen intake, he developed symptoms of the gastrointestinal tract, with approximately ten water diarrheas per day. In complete blood count and liver function tests, eosinophilia with 1,638x109/l (21% of 7,8x109/l WBC) and elevated liver enzymes were found. Parasitological examination of the stool revealed the mass of pollen grains of various plants with a large number of Charcot-Leyden crystals. It was concluded that the patient was sensitive to pollen and was advised to stop the self-medication.}, keywords = {diarrhea, Pollen, pollen grains, sensitization}, pubstate = {published}, tppubtype = {article} } A 62-year-old patient, in good condition, reported to the doctor for vomiting and diarrhea. A month after the beginning of oral bee pollen intake, he developed symptoms of the gastrointestinal tract, with approximately ten water diarrheas per day. In complete blood count and liver function tests, eosinophilia with 1,638x109/l (21% of 7,8x109/l WBC) and elevated liver enzymes were found. Parasitological examination of the stool revealed the mass of pollen grains of various plants with a large number of Charcot-Leyden crystals. It was concluded that the patient was sensitive to pollen and was advised to stop the self-medication. |
| Banović, Pavle; Stankov, Srđan; Vranješ, Nenad; Zurković, Ognjen; Čapo, Ivan; Lalošević, Dušan Drug repurposing: mebendazole as effective antitumor agent. Are we seeing the whole story? Journal Article JBUON, 23 (6), pp. 1904-1911, 2018, ISSN: 1107-0625, online ISSN: 2241-6293. Abstract | Links | BibTeX | Tags: BHK21 cells, drug repurposing, fibrosarcoma, hamster, mebendazole, tumor @article{JBUON2018;23(6):1911, title = {Drug repurposing: mebendazole as effective antitumor agent. Are we seeing the whole story?}, author = {Pavle Banović and Srđan Stankov and Nenad Vranješ and Ognjen Zurković and Ivan Čapo and Dušan Lalošević}, url = {https://www.pasterovzavod.rs/wp-content/uploads/2019/02/23-6-1904-1911-2.pdf}, issn = {1107-0625, online ISSN: 2241-6293}, year = {2018}, date = {2018-06-09}, journal = {JBUON}, volume = {23}, number = {6}, pages = {1904-1911}, institution = {Medical Faculty Novi Sad, University of Novi Sad, 21000 Novi Sad, Serbia; Medical Faculty Novi Sad, University of Novi Sad, Department of Histology and Embryology, 21000 Novi Sad, Serbia; Pasteur Institute Novi Sad, 21000 Novi Sad, Serbia}, abstract = {Purpose: To examine the antitumor effects of Mebendazole (MZ) in a model of experimental fibrosarcoma induced by inoculation of BHK-21/C13 cells in Syrian golden hamster. Methods: Hamsters were inoculated with a suspension of BHK cells by subcutaneous injection and randomly divided into 5 experimental and 2 control groups. Treatment started on the 10th day after inoculation, when the tumor grew to a diameter of 5mm. The experimental design was based on distributing the total amount of drug MZ(z) in different protocols and approaches (oral/intraperitoneal) to the 5 experimental groups. The positive control group received doxorubicin intraperitoneally. Negative control group received olive oil orally. The total amount of MZ(z) was chosen to be the highest for the animal to survive during the experiment. For antitumor effect evaluation, the main parameters were tu mor size, number of mitoses, cytochrome-C immunopositivity and tumor tissue morphology incuding cytoarchitecture and percentage of preserved tumor tissue in stereologically reconstructed tumor mass. Results: The results of this study showed absence of objective MZ antitumor effect on experimental fibrosarcoma. MZ does not exhibit activity similar to DNA-damaging agents on the fibrosarcoma model. Conclusions: It might be postulated that soft tissue tumors on animal models could show high level of resistance to MZ effect. }, keywords = {BHK21 cells, drug repurposing, fibrosarcoma, hamster, mebendazole, tumor}, pubstate = {published}, tppubtype = {article} } Purpose: To examine the antitumor effects of Mebendazole (MZ) in a model of experimental fibrosarcoma induced by inoculation of BHK-21/C13 cells in Syrian golden hamster. Methods: Hamsters were inoculated with a suspension of BHK cells by subcutaneous injection and randomly divided into 5 experimental and 2 control groups. Treatment started on the 10th day after inoculation, when the tumor grew to a diameter of 5mm. The experimental design was based on distributing the total amount of drug MZ(z) in different protocols and approaches (oral/intraperitoneal) to the 5 experimental groups. The positive control group received doxorubicin intraperitoneally. Negative control group received olive oil orally. The total amount of MZ(z) was chosen to be the highest for the animal to survive during the experiment. For antitumor effect evaluation, the main parameters were tu mor size, number of mitoses, cytochrome-C immunopositivity and tumor tissue morphology incuding cytoarchitecture and percentage of preserved tumor tissue in stereologically reconstructed tumor mass. Results: The results of this study showed absence of objective MZ antitumor effect on experimental fibrosarcoma. MZ does not exhibit activity similar to DNA-damaging agents on the fibrosarcoma model. Conclusions: It might be postulated that soft tissue tumors on animal models could show high level of resistance to MZ effect. |
| Čabrilo, Olivera Bjelić; Simin, Verica; Miljević, Vilan; Čabrilo, Borislav; Mijatović, Dragana; Lalošević, Dušan Respiratory and cardiopulmonary nematode species of foxes and jackals in Serbia Journal Article SCIENDO / Helminthologia, 5 , pp. 213–221, 2018, ISSN: 3. Abstract | Links | BibTeX | Tags: cardiopulmonary nematodes, jackal, red fox, respiratory nematodes, Serbia @article{Helminthologia, title = {Respiratory and cardiopulmonary nematode species of foxes and jackals in Serbia}, author = {Olivera Bjelić Čabrilo and Verica Simin and Vilan Miljević and Borislav Čabrilo and Dragana Mijatović and Dušan Lalošević}, url = {https://www.pasterovzavod.rs/wp-content/uploads/2019/02/13369083-Helminthologia-Respiratory-and-cardiopulmonary-nematode-species-of-foxes-and-jackals-in-Serbia.pdf}, doi = {10.2478/helm-2018-0019}, issn = {3}, year = {2018}, date = {2018-05-07}, journal = {SCIENDO / Helminthologia}, volume = {5}, pages = {213–221}, abstract = {As part of routine monitoring of foxes (Vulpes vulpes) and jackals (Canis aureus) on the territory of Vojvodina province (northern Serbia), an analysis of respiratory and cardiopulmonary parasitic nematodes was conducted. Both host species harbored Eucoleus aerophilus, E. boehmi and Crenosoma vulpis, whereas Angiostrongylus vasorum was found only in foxes. A high prevalence of infection (72.6 %) was noted for E. aerophilus in foxes. The remaining parasite species occurred less frequently in both host species. In all species where it could be quantifi ed, a high degree of parasite aggregation within host individuals was noted. Single species infections were most common, whereas two and three species infections occurred less frequently in both host species. The distribution of abundance of E. aerophilus was affected by host sex, with abundances higher in male foxes. Sampling site and year infl uenced abundance variation in E. boehmi. }, keywords = {cardiopulmonary nematodes, jackal, red fox, respiratory nematodes, Serbia}, pubstate = {published}, tppubtype = {article} } As part of routine monitoring of foxes (Vulpes vulpes) and jackals (Canis aureus) on the territory of Vojvodina province (northern Serbia), an analysis of respiratory and cardiopulmonary parasitic nematodes was conducted. Both host species harbored Eucoleus aerophilus, E. boehmi and Crenosoma vulpis, whereas Angiostrongylus vasorum was found only in foxes. A high prevalence of infection (72.6 %) was noted for E. aerophilus in foxes. The remaining parasite species occurred less frequently in both host species. In all species where it could be quantifi ed, a high degree of parasite aggregation within host individuals was noted. Single species infections were most common, whereas two and three species infections occurred less frequently in both host species. The distribution of abundance of E. aerophilus was affected by host sex, with abundances higher in male foxes. Sampling site and year infl uenced abundance variation in E. boehmi. |
| Suli, Tamás; Kozderović, Gordana; Potkonjak, Aleksandar; Simin, Stanislav; Simin, Verica; Lalošević, Vesna Comparison of Conventional and Molecular Diagnostic Techniques for Detection of Blastocystis sp. in Pig Faeces Journal Article Iranian Journal of Parasitology, 13 (4), pp. 594-601, 2018. Abstract | Links | BibTeX | Tags: @article{article, title = {Comparison of Conventional and Molecular Diagnostic Techniques for Detection of Blastocystis sp. in Pig Faeces}, author = {Tamás Suli and Gordana Kozderović and Aleksandar Potkonjak and Stanislav Simin and Verica Simin and Vesna Lalošević}, url = {https://www.pasterovzavod.rs/wp-content/uploads/2019/02/1686-1.pdf}, year = {2018}, date = {2018-03-19}, journal = {Iranian Journal of Parasitology}, volume = {13}, number = {4}, pages = {594-601}, abstract = {Background: Blastocystis is a common protist colonizing the gastrointestinal tract of humans and various animals. Pigs have been suggested to be a reservoir for human Blastocystis infections because of high prevalence of the parasite in these animals and the presence of zoonotic subtypes. Nevertheless, epidemiological data is often misinterpreted due to the lack of standard diagnostic procedures. This study aimed to compare the sensitivity of different diagnostic techniques in detection of Blastocystis sp. in pigs. Methods: Overall, 48 individual faecal samples were collected from pigs reared in an intensive farming system (Autonomous Province of Vojvodina, Serbia) and were tested by microscopic examination of direct wet mount, in vitro cultivation in modified Jones' medium and conventional PCR for rRNA gene. Results: Xenic in vitro cultivation in Jones’ medium showed higher sensitivity than direct wet mount when we compared it with PCR. Namely, the estimated sensitivity of direct wet mount was 46.15%, while the sensitivity of in vitro cultivation was 84.62%. Conclusion: Low sensitivity of conventional parasitological compared to molecular methods is proven. Thus, reports on prevalence that rely solely on microscopy of faecal samples (unprocessed or concentrated) are probably underestimating the true prevalence of the parasite.}, keywords = {}, pubstate = {published}, tppubtype = {article} } Background: Blastocystis is a common protist colonizing the gastrointestinal tract of humans and various animals. Pigs have been suggested to be a reservoir for human Blastocystis infections because of high prevalence of the parasite in these animals and the presence of zoonotic subtypes. Nevertheless, epidemiological data is often misinterpreted due to the lack of standard diagnostic procedures. This study aimed to compare the sensitivity of different diagnostic techniques in detection of Blastocystis sp. in pigs. Methods: Overall, 48 individual faecal samples were collected from pigs reared in an intensive farming system (Autonomous Province of Vojvodina, Serbia) and were tested by microscopic examination of direct wet mount, in vitro cultivation in modified Jones' medium and conventional PCR for rRNA gene. Results: Xenic in vitro cultivation in Jones’ medium showed higher sensitivity than direct wet mount when we compared it with PCR. Namely, the estimated sensitivity of direct wet mount was 46.15%, while the sensitivity of in vitro cultivation was 84.62%. Conclusion: Low sensitivity of conventional parasitological compared to molecular methods is proven. Thus, reports on prevalence that rely solely on microscopy of faecal samples (unprocessed or concentrated) are probably underestimating the true prevalence of the parasite. |
| Cimpean, Anca Maria; Lalošević, Dušan; Lalošević, Vesna; Banović, Pavle; Raica, Marius; Mederle, Ovidiu Alexandru In Vivo, 32 (4), pp. 791-798, 2018. Abstract | Links | BibTeX | Tags: @article{cimpean2018, title = {Disodium Cromolyn and Anti-podoplanin Antibodies Strongly Inhibit Growth of BHK 21/C13-derived Fibrosarcoma in a Chick Embryo Chorioallantoic Membrane Model }, author = {Anca Maria Cimpean and Dušan Lalošević and Vesna Lalošević and Pavle Banović and Marius Raica and Ovidiu Alexandru Mederle}, url = {https://www.pasterovzavod.rs/wp-content/uploads/2019/02/cimpean2018.pdf}, doi = {10.21873/invivo.11309}, year = {2018}, date = {2018-03-04}, journal = {In Vivo}, volume = {32}, number = {4}, pages = {791-798}, institution = {Department of Microscopic Morphology/Histology, Angiogenesis Research Center, Victor Babes University of Medicine and Pharmacy, Timisoara, Romania; Department of Histology and Embryology, Medical Faculty, and Department of Veterinary Medicine, Faculty of Agriculture, University of Novi Sad, Novi Sad, Serbia}, abstract = {AIM: To characterize baby hamster kidney fibroblast (BHK 21/C13) cells and test the effects of antibodies against podoplanin and disodium cromolyn on BHK 21/C13 cell line-derived tumors grown on chick embryo chorioallantoic membrane (CAM). MATERIAL AND METHODS: BHK 21/C13 cell-derived fibrosarcomas developed in hamsters were implanted on CAM and treated with anti-podoplanin antibodies and disodium cromolyn. BHK 21/C13 cell immunophenotype was assessed. RESULTS: Fibrosarcoma cells were positive for vimentin, CD117, smooth muscle actin, vascular endothelial growth factor epidermal growth factor receptor, homebox prospero gene 1 and negative for platelet-derived growth factor B, neuron-specific enolase, S100, CD34, Ewing sarcoma and podoplanin. CAM-grown fibrosarcomas were highly sensitive to disodium cromolyn and anti-podoplanin antibodies. CONCLUSION: Immunophenotyping BHK 21/C13 cells and their response to drugs represent the first step in revealing cell line utility and a reliable tool for experimental cancer research.}, keywords = {}, pubstate = {published}, tppubtype = {article} } AIM: To characterize baby hamster kidney fibroblast (BHK 21/C13) cells and test the effects of antibodies against podoplanin and disodium cromolyn on BHK 21/C13 cell line-derived tumors grown on chick embryo chorioallantoic membrane (CAM). MATERIAL AND METHODS: BHK 21/C13 cell-derived fibrosarcomas developed in hamsters were implanted on CAM and treated with anti-podoplanin antibodies and disodium cromolyn. BHK 21/C13 cell immunophenotype was assessed. RESULTS: Fibrosarcoma cells were positive for vimentin, CD117, smooth muscle actin, vascular endothelial growth factor epidermal growth factor receptor, homebox prospero gene 1 and negative for platelet-derived growth factor B, neuron-specific enolase, S100, CD34, Ewing sarcoma and podoplanin. CAM-grown fibrosarcomas were highly sensitive to disodium cromolyn and anti-podoplanin antibodies. CONCLUSION: Immunophenotyping BHK 21/C13 cells and their response to drugs represent the first step in revealing cell line utility and a reliable tool for experimental cancer research. |
2017 |
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| Banović, Pavle TREATMENT OF A PATIENT INJURED BY THE CENTIPEDE SCOLOPENDRA CINGULATA (LATERILLE, 1829) Journal Article MD-Medical Data, 9 (3), pp. 185-187, 2017. Abstract | Links | BibTeX | Tags: ScoLopendra cinGULata, scolopendrism, Serbia, treatment @article{article, title = {TREATMENT OF A PATIENT INJURED BY THE CENTIPEDE SCOLOPENDRA CINGULATA (LATERILLE, 1829)}, author = {Pavle Banović}, url = {https://www.pasterovzavod.rs/wp-content/uploads/2019/02/09-MD-Vol-9-No3_Banovic-5-1.pdf}, year = {2017}, date = {2017-09-13}, journal = {MD-Medical Data}, volume = {9}, number = {3}, pages = {185-187}, abstract = {Scolopendra cingulata (Laterille, 1829) was noticed sixty years ago for the first time in one location in Serbia and since then data about it are scarce. It represents the biggest centipede in Europe and only one that can seriously harm humans. Scolopendrism in Republic of Serbia is a true rarity and until now there has been no case report of harming human by centipede Scolopendra cingulata (Laterille, 1829). Patient aged 85, was stung on his right leg toe by unknown centipede in the toilet of his home. The centipede was afterwards identified as Scolopendra cingulata. As a chief complaint patient describes intense pain at the sting site and burning feeling that spreads to the hip joint. The objective finding revealed a slight redness of the affected area. He was ordinated 20mg chloropyramine (Synopen), 2x4mg dexamethasone and 40mg methylprednisolone i.v. 24 hours after centipede attack pain was completely gone and the patient could walk freely. Although there is a possibility that a species located in our country's territory exhibits phenotypes and genotype differences in relation to the more widely described Mediterranean type, each human bitten by i.e. that come into contact with Scolopendra cingulata venom should be taken seriously in order to facilitate the patient's symptoms, manage constant monitoring and prevent the development of isolated or associated complications with potential comorbidities of the patient.}, keywords = {ScoLopendra cinGULata, scolopendrism, Serbia, treatment}, pubstate = {published}, tppubtype = {article} } Scolopendra cingulata (Laterille, 1829) was noticed sixty years ago for the first time in one location in Serbia and since then data about it are scarce. It represents the biggest centipede in Europe and only one that can seriously harm humans. Scolopendrism in Republic of Serbia is a true rarity and until now there has been no case report of harming human by centipede Scolopendra cingulata (Laterille, 1829). Patient aged 85, was stung on his right leg toe by unknown centipede in the toilet of his home. The centipede was afterwards identified as Scolopendra cingulata. As a chief complaint patient describes intense pain at the sting site and burning feeling that spreads to the hip joint. The objective finding revealed a slight redness of the affected area. He was ordinated 20mg chloropyramine (Synopen), 2x4mg dexamethasone and 40mg methylprednisolone i.v. 24 hours after centipede attack pain was completely gone and the patient could walk freely. Although there is a possibility that a species located in our country's territory exhibits phenotypes and genotype differences in relation to the more widely described Mediterranean type, each human bitten by i.e. that come into contact with Scolopendra cingulata venom should be taken seriously in order to facilitate the patient's symptoms, manage constant monitoring and prevent the development of isolated or associated complications with potential comorbidities of the patient. |
| Ristanović, Elizabeta; Atanasievska, Sonja; Protić-Đokić, Vesna; Hinić, Nataša; Lalošević, Dušan MICROBIOLOGICAL DETECTION OF BORRELIA BURGDORFERI AND CONTEMPORARY DIAGNOSIS OF LYME BORELLOSIS Journal Article MD Medical review, 9 (3), pp. 143-147, 2017. Abstract | Links | BibTeX | Tags: Borrelia burgdorferi, Lyme borreliosis, PCR method, Serological tests @article{article, title = {MICROBIOLOGICAL DETECTION OF BORRELIA BURGDORFERI AND CONTEMPORARY DIAGNOSIS OF LYME BORELLOSIS}, author = {Elizabeta Ristanović and Sonja Atanasievska and Vesna Protić-Đokić and Nataša Hinić and Dušan Lalošević}, url = {https://www.pasterovzavod.rs/wp-content/uploads/2019/02/01-MD-Vol-9-No3_Ristanovic-et-al.pdf}, year = {2017}, date = {2017-08-24}, journal = {MD Medical review}, volume = {9}, number = {3}, pages = {143-147}, abstract = {Lyme borreliosis is a multi-system disease caused by Borrelia burgdorferi, which is transmitted by hard ticks Ixodes spp. The most common clinical manifestation is the skin lesion (erythema migrans), however there is a tendency of the pathogen to spread and cause damages to the joints, nervous and cardiovascular system. The diagnosis of Lyme disease is based primarily on clinical findings, epidemiological and anamnestic data and laboratory test results. The application of the modern real-time PCR method allows us to test the skin in tick bite area for a presence of B. burgdorferi. In the diagnosis of Lyme disease, we often use indirect methods (TIIF, ELISA, Western blot) for detecting specific IgM and IgG antibodies against B. burgdorferi in serum, liquor and synovial fluid. To confirm the diagnosis, the presence of IgG antibodies should be demonstrated, since the presence of IgM is not a relible indicator of a recent infection. When interpreting immunodiagnostic test results it has to be considered that there are a lot of variability as the phenotype characteristics of borrelia, different antigenic structures, different geographical distribution, phase varies in duration of the disease, characteristics of individual immune responses, presence of other diseases as well as the applied antibiotic treatment, which all can affect the outcome. Due to the high presence of infected ticks in our geographical area, it is necessary to provide continuous preventive measures, epidemiological monitoring and improvement of the diagnostic and therapeutic approach in the treatment of Lyme borreliosis.}, keywords = {Borrelia burgdorferi, Lyme borreliosis, PCR method, Serological tests}, pubstate = {published}, tppubtype = {article} } Lyme borreliosis is a multi-system disease caused by Borrelia burgdorferi, which is transmitted by hard ticks Ixodes spp. The most common clinical manifestation is the skin lesion (erythema migrans), however there is a tendency of the pathogen to spread and cause damages to the joints, nervous and cardiovascular system. The diagnosis of Lyme disease is based primarily on clinical findings, epidemiological and anamnestic data and laboratory test results. The application of the modern real-time PCR method allows us to test the skin in tick bite area for a presence of B. burgdorferi. In the diagnosis of Lyme disease, we often use indirect methods (TIIF, ELISA, Western blot) for detecting specific IgM and IgG antibodies against B. burgdorferi in serum, liquor and synovial fluid. To confirm the diagnosis, the presence of IgG antibodies should be demonstrated, since the presence of IgM is not a relible indicator of a recent infection. When interpreting immunodiagnostic test results it has to be considered that there are a lot of variability as the phenotype characteristics of borrelia, different antigenic structures, different geographical distribution, phase varies in duration of the disease, characteristics of individual immune responses, presence of other diseases as well as the applied antibiotic treatment, which all can affect the outcome. Due to the high presence of infected ticks in our geographical area, it is necessary to provide continuous preventive measures, epidemiological monitoring and improvement of the diagnostic and therapeutic approach in the treatment of Lyme borreliosis. |
2016 |
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| Lalošević, Dušan; Lalošević, Vesna; Simin, Verica; Miljević, Milan; Čabrilo, Borislav; Čabrilo, Olivera Bjelić Spreading of multilocular echinococcosis in southern Europe: 5 the first record in foxes and jackals in Serbia, Vojvodina Province Journal Article European Journal of Wildlife Research, 62 (6), pp. 793-796, 2016, ISSN: 1612-4642. Abstract | Links | BibTeX | Tags: carnivores, echinococcosis, Echinococcus multilocularis Search subject for Echinococcus multilocularis, foxes, intestines, jackals, monitoring, parasites, Rabies, Serbia, Southern European region @article{article, title = {Spreading of multilocular echinococcosis in southern Europe: 5 the first record in foxes and jackals in Serbia, Vojvodina Province}, author = {Dušan Lalošević and Vesna Lalošević and Verica Simin and Milan Miljević and Borislav Čabrilo and Olivera Bjelić Čabrilo}, url = {https://www.pasterovzavod.rs/wp-content/uploads/2019/02/Lalosevic-D.-et-al_Spreading-of-echinococcosis_Author-uncorrected.pdf}, doi = {10.1007/s10344-016-1050-9}, issn = {1612-4642}, year = {2016}, date = {2016-09-13}, journal = {European Journal of Wildlife Research}, volume = {62}, number = {6}, pages = {793-796}, abstract = {Foxes and jackals from the Vojvodina Province of Serbia were examined for the presence of echinococcosis. Animals were collected as part of routine rabies monitoring and autopsied; their intestines were checked for parasites. Out of 112 examined foxes, echinococcosis was found in 20 (17.9 %); of 28 examined jackals, 4 were infected (14.3 %). Morphological analysis confirmed the presence of Echinococcus multilocularis. This is the first record of E. multilocularis in foxes or other carnivorous mammals in Serbia.}, keywords = {carnivores, echinococcosis, Echinococcus multilocularis Search subject for Echinococcus multilocularis, foxes, intestines, jackals, monitoring, parasites, Rabies, Serbia, Southern European region}, pubstate = {published}, tppubtype = {article} } Foxes and jackals from the Vojvodina Province of Serbia were examined for the presence of echinococcosis. Animals were collected as part of routine rabies monitoring and autopsied; their intestines were checked for parasites. Out of 112 examined foxes, echinococcosis was found in 20 (17.9 %); of 28 examined jackals, 4 were infected (14.3 %). Morphological analysis confirmed the presence of Echinococcus multilocularis. This is the first record of E. multilocularis in foxes or other carnivorous mammals in Serbia. |
2014 |
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| AIKIMBAYEV, Alim; BRIGGS, Deborah; COLTAN, Gabriel; Dodet, Betty; Farahtaj, Firouzeh; IMNADZE, Paata; KOREJWO, Joanna; MOISEIEVA, Anna; Usluer, Gaye; Tordo, Noel; Vodopija, Rodovan; Vranješ, Nenad Fighting Rabies in Eastern Europe, the Middle East and Central Asia - Experts Call for a Regional Initiative for Rabies Elimination Journal Article Zoonoses and Public Health, pp. 219–226, 2014. Abstract | Links | BibTeX | Tags: public health programmes, Rabies, rabies control, zoonosis @article{article, title = {Fighting Rabies in Eastern Europe, the Middle East and Central Asia - Experts Call for a Regional Initiative for Rabies Elimination}, author = {Alim AIKIMBAYEV and Deborah BRIGGS and Gabriel COLTAN and Betty Dodet and Firouzeh Farahtaj and Paata IMNADZE and Joanna KOREJWO and Anna MOISEIEVA and Gaye Usluer and Noel Tordo and Rodovan Vodopija and Nenad Vranješ}, url = {https://www.pasterovzavod.rs/wp-content/uploads/2019/02/aikimbayev2013.pdf}, doi = {10.1111/zph.12060}, year = {2014}, date = {2014-05-00}, journal = {Zoonoses and Public Health}, pages = {219–226}, abstract = {MEEREB is an informal network of rabies experts from the Middle East, Eastern Europe and Central Asia, seeking to eliminate rabies from the region. They met for the second time to review the current rabies situation, both globally and in their respective countries, highlighting current rabies control problems and potential solutions. Success stories in Latin America, in Western Europe, in some Asian countries, as well as in Croatia and Serbia prove that elimination of human rabies is achievable in the MEEREB region. It requires political willingness and cooperation of all stakeholders, including Ministries of Health and of Agriculture; adequate management of animal bites through post-exposure prophylaxis; pre-exposure prophylaxis for populations at high risk of rabies exposure, animal vaccination and humane control of stray dog populations. MEEREB members called for a regional initiative for rabies elimination in Eastern Europe and the Middle East. They are confident that the elimination of human rabies of canine origin can be achieved in the region through adopting a One Health approach, and that campaigns for rabies elimination will have significant benefit for public health, including strengthening the structure for control of other zoonoses.}, keywords = {public health programmes, Rabies, rabies control, zoonosis}, pubstate = {published}, tppubtype = {article} } MEEREB is an informal network of rabies experts from the Middle East, Eastern Europe and Central Asia, seeking to eliminate rabies from the region. They met for the second time to review the current rabies situation, both globally and in their respective countries, highlighting current rabies control problems and potential solutions. Success stories in Latin America, in Western Europe, in some Asian countries, as well as in Croatia and Serbia prove that elimination of human rabies is achievable in the MEEREB region. It requires political willingness and cooperation of all stakeholders, including Ministries of Health and of Agriculture; adequate management of animal bites through post-exposure prophylaxis; pre-exposure prophylaxis for populations at high risk of rabies exposure, animal vaccination and humane control of stray dog populations. MEEREB members called for a regional initiative for rabies elimination in Eastern Europe and the Middle East. They are confident that the elimination of human rabies of canine origin can be achieved in the region through adopting a One Health approach, and that campaigns for rabies elimination will have significant benefit for public health, including strengthening the structure for control of other zoonoses. |
2013 |
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| Lalošević, Vesna; Lalošević, Dušan; Čapo, Ivan; Simin, Verica; Galfi, Annamaria; Traversa, Donato High infection rate of zoonotic Eucoleus aerophilus infection in foxes from Serbia Journal Article Parasite, 20 (3), pp. 1-6, 2013. Abstract | Links | BibTeX | Tags: Eucoleus aerophilus, Eucoleus boehmi, red fox @article{article, title = {High infection rate of zoonotic Eucoleus aerophilus infection in foxes from Serbia}, author = {Vesna Lalošević and Dušan Lalošević and Ivan Čapo and Verica Simin and Annamaria Galfi and Donato Traversa}, url = {https://www.pasterovzavod.rs/wp-content/uploads/2019/02/10.1051@parasite@2012003.pdf}, doi = {10.1051/parasite/2012003}, year = {2013}, date = {2013-01-14}, journal = {Parasite}, volume = {20}, number = {3}, pages = {1-6}, abstract = {The respiratory capillariid nematode Eucoleus aerophilus (Creplin, 1839) infects wild and domestic carnivores and, occasionally, humans. Thus far, a dozen of human infections have been published in the literature but it cannot be ruled out that lung capillariosis is underdiagnosed in human medicine. Also, the apparent spreading of E. aerophilus in different geographic areas spurs new studies on the epidemiology of this nematode. After the recognition of the first human case of E. aerophilus infection in Serbia, there is a significant merit in enhancing knowledge on the distribution of the nematode. In the present work the infection rate of pulmonary capillariosis was investigated in 70 red foxes (Vulpes vulpes) from the northern part of Serbia by autopsy. The estimated infection rate with Eucoleus aerophilus was 84%. In contrast, by copromicroscopic examination only 38% of foxes were positive. In addition, 10 foxes were investigated for the closely related species in nasal cavity, Eucoleus boehmi, and nine were positive. Our study demonstrates one of the highest infection rates of pulmonary capillariosis in foxes over the world.}, keywords = {Eucoleus aerophilus, Eucoleus boehmi, red fox}, pubstate = {published}, tppubtype = {article} } The respiratory capillariid nematode Eucoleus aerophilus (Creplin, 1839) infects wild and domestic carnivores and, occasionally, humans. Thus far, a dozen of human infections have been published in the literature but it cannot be ruled out that lung capillariosis is underdiagnosed in human medicine. Also, the apparent spreading of E. aerophilus in different geographic areas spurs new studies on the epidemiology of this nematode. After the recognition of the first human case of E. aerophilus infection in Serbia, there is a significant merit in enhancing knowledge on the distribution of the nematode. In the present work the infection rate of pulmonary capillariosis was investigated in 70 red foxes (Vulpes vulpes) from the northern part of Serbia by autopsy. The estimated infection rate with Eucoleus aerophilus was 84%. In contrast, by copromicroscopic examination only 38% of foxes were positive. In addition, 10 foxes were investigated for the closely related species in nasal cavity, Eucoleus boehmi, and nine were positive. Our study demonstrates one of the highest infection rates of pulmonary capillariosis in foxes over the world. |
2012 |
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| Stankov, Srđan Definition of Inflammation, Causes of Inflammation and Possible Anti-inflammatory Strategies Journal Article The Open Inflammation Journal, 5 , pp. 1-9, 2012. Abstract | Links | BibTeX | Tags: anti-inflammatory response, burns, catabolism, edema, Inflammation, osmotic pressure @article{article, title = {Definition of Inflammation, Causes of Inflammation and Possible Anti-inflammatory Strategies}, author = {Srđan Stankov}, url = {https://www.pasterovzavod.rs/wp-content/uploads/2019/02/Stankov-1.pdf}, doi = {10.2174/1875041901205010001}, year = {2012}, date = {2012-07-15}, journal = {The Open Inflammation Journal}, volume = {5}, pages = {1-9}, institution = {Pasteur Institute Novi Sad, Hajduk Veljkova 1, 21000 Novi Sad, Serbia}, abstract = {Current definition of inflammation by its cardinal signs is obsolete and unsuitable for guiding adequate therapeutic strategies. Furthermore, present theory of the inflammatory process regarding vascular phenomena as essential for generation of cardinal signs is invalid and unable to explain well established empirical facts, particularly the extent of the osmotic pressure and temperature variations within the inflamed tissue. From five cardinal signs, there is actually just one specific macroscopic sign of inflammation, namely localized edema. Further, the driving force for tissue fluid accumulation is defined in biochemical terms and as such taken for the definition of the inflammatory process. Inflammation may be defined as a degenerative process which is intense enough to cause local accumulation of low molecular weight catabolic products, which in turn elevates tissue osmotic pressure that attracts extra fluid, with or without heat release sufficient for significant elevation of tissue temperature. This process is in a sharp contrast to the pathogenesis of burns, where externally applied heat causes a process that is in essence opposite to inflammation, bearing only some superficial similarities with the latter. The inflammatory process is itself a pathological process, whereas the natural anti-inflammatory response that ensues after acute inflammation tends to reverse tissue homeostasis towards normality and should therefore be regarded as a true defensive reaction of the affected tissue. Based on the therapeutic principle of reverse thermodynamics, heat application to the inflamed tissue is an obvious, yet non-exclusive therapeutic choice that follows from the given universal definition of inflammation. }, keywords = {anti-inflammatory response, burns, catabolism, edema, Inflammation, osmotic pressure}, pubstate = {published}, tppubtype = {article} } Current definition of inflammation by its cardinal signs is obsolete and unsuitable for guiding adequate therapeutic strategies. Furthermore, present theory of the inflammatory process regarding vascular phenomena as essential for generation of cardinal signs is invalid and unable to explain well established empirical facts, particularly the extent of the osmotic pressure and temperature variations within the inflamed tissue. From five cardinal signs, there is actually just one specific macroscopic sign of inflammation, namely localized edema. Further, the driving force for tissue fluid accumulation is defined in biochemical terms and as such taken for the definition of the inflammatory process. Inflammation may be defined as a degenerative process which is intense enough to cause local accumulation of low molecular weight catabolic products, which in turn elevates tissue osmotic pressure that attracts extra fluid, with or without heat release sufficient for significant elevation of tissue temperature. This process is in a sharp contrast to the pathogenesis of burns, where externally applied heat causes a process that is in essence opposite to inflammation, bearing only some superficial similarities with the latter. The inflammatory process is itself a pathological process, whereas the natural anti-inflammatory response that ensues after acute inflammation tends to reverse tissue homeostasis towards normality and should therefore be regarded as a true defensive reaction of the affected tissue. Based on the therapeutic principle of reverse thermodynamics, heat application to the inflamed tissue is an obvious, yet non-exclusive therapeutic choice that follows from the given universal definition of inflammation. |
| Simin, Verica; Lalošević, Vesna; Galfi, Annamaria; Božić, Milovan; Obradović, Nemanja; Lalošević, Dušan Crenosoma vulpis (Dujardin 1844) (Nematoda, Crenosomatidae) in foxes in Vojvodina Province, Serbia Journal Article BiologiaSerbica, 34 (1-2), pp. 71-74, 2012. Abstract | Links | BibTeX | Tags: Crenosoma vulpis, foxes, prevalence, Serbia, Vojvodina @article{article, title = {Crenosoma vulpis (Dujardin 1844) (Nematoda, Crenosomatidae) in foxes in Vojvodina Province, Serbia}, author = {Verica Simin and Vesna Lalošević and Annamaria Galfi and Milovan Božić and Nemanja Obradović and Dušan Lalošević}, url = {https://www.pasterovzavod.rs/wp-content/uploads/2019/02/1282-2342-1-PB-2.pdf}, year = {2012}, date = {2012-03-13}, journal = {BiologiaSerbica}, volume = {34}, number = {1-2}, pages = {71-74}, institution = {Pasteur Institute of Novi Sad, Hajduk Veljkova, 21000 Novi Sad, Serbia, Department of Veterinary Medicine, Faculty of Agriculture, University of Novi Sad, Trg Dositeja Obradovića 8, 21000 Novi Sad, Serbia, Faculty of Medicine, University of Novi Sad, Hajduk Veljkova 3, 21000 Novi Sad, Serbia}, abstract = {Crenosoma vulpis is a lung worm which aff ects carnivorous mammals. Data on the presence of C. vulpis in foxes in Serbia is lacking. In the present study, C. vulpis was found in the fox population of Vojvodina province, Serbia, during the course of examinations for Eucoleus aerophilus. Foxes were collected throughout Vojvodina province: most of the foxes had either been previously suspected of rabies or regularly shot in 2011. carcasses were opened and tracheas were taken from larynx to bifurcation, and preserved in 30% ethanol for later examination. For the purpose of examination, the tracheas were opened on the anterior side using scissors. parasites were collected under a stereomicroscope by scraping of mucosa and wet-mounted in glycerin-ethanol or lactophenol. Crenosoma vulpis was found in 5 out of 38 foxes, for a prevalence rate of 13.15%. a total of 8 male, 15 female and 45 larvae was found. cohabitation of foxes with stray dogs and cats may be a potential source of parasite transmission in urban environments. }, keywords = {Crenosoma vulpis, foxes, prevalence, Serbia, Vojvodina}, pubstate = {published}, tppubtype = {article} } Crenosoma vulpis is a lung worm which aff ects carnivorous mammals. Data on the presence of C. vulpis in foxes in Serbia is lacking. In the present study, C. vulpis was found in the fox population of Vojvodina province, Serbia, during the course of examinations for Eucoleus aerophilus. Foxes were collected throughout Vojvodina province: most of the foxes had either been previously suspected of rabies or regularly shot in 2011. carcasses were opened and tracheas were taken from larynx to bifurcation, and preserved in 30% ethanol for later examination. For the purpose of examination, the tracheas were opened on the anterior side using scissors. parasites were collected under a stereomicroscope by scraping of mucosa and wet-mounted in glycerin-ethanol or lactophenol. Crenosoma vulpis was found in 5 out of 38 foxes, for a prevalence rate of 13.15%. a total of 8 male, 15 female and 45 larvae was found. cohabitation of foxes with stray dogs and cats may be a potential source of parasite transmission in urban environments. |
2011 |
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| McElhinney, L M; Marston, D A; Freuling, C M; Cragg, W; Stankov, Srđan; Lalošević, Dušan; Müller, T; Fooks, A R Molecular diversity and evolutionary history of rabies virus strains circulating in the Balkans Journal Article Journal of General Virology, (92), pp. 2171–2180, 2011. Abstract | Links | BibTeX | Tags: Rabies, rabies virus (RABV), Rabv @article{article, title = {Molecular diversity and evolutionary history of rabies virus strains circulating in the Balkans}, author = {L. M. McElhinney and D. A. Marston and C. M. Freuling and W. Cragg and Srđan Stankov and Dušan Lalošević and T. Müller and A. R. Fooks}, url = {https://www.pasterovzavod.rs/wp-content/uploads/2019/02/Stankov.pdf}, doi = {10.1099/vir.0.032748-0}, year = {2011}, date = {2011-05-31}, journal = {Journal of General Virology}, number = {92}, pages = {2171–2180}, abstract = {Molecular studies of European classical rabies viruses (RABV) have revealed a number of geographically clustered lineages. To study the diversity of Balkan RABV, partial nucleoprotein (N) gene sequences were analysed from a unique panel of isolates (n5210), collected from various hosts between 1972 and 2006. All of the Balkan isolates grouped within the European/Middle East Lineage, with the majority most closely related to East European strains. A number of RABV from Bosnia & Herzegovina and Montenegro, collected between 1986 and 2006, grouped with the West European strains, believed to be responsible for the rabies epizootic that spread throughout Europe in the latter half of the 20th Century. In contrast, no Serbian RABV belonged to this sublineage. However, a distinct group of Serbian fox RABV provided further evidence for the southwards wildlife-mediated movement of rabies from Hungary, Romania and Serbia into Bulgaria. To determine the optimal region for evolutionary analysis, partial, full and concatenated N-gene and glycoprotein (G) gene sequences were compared. Whilst both the divergence times and evolutionary rates were similar irrespective of genomic region, the 95% highest probability density (HPD) limits were significantly reduced for full N-gene and concatenated NG-gene sequences compared with partial gene sequences. Bayesian coalescent analysis estimated the date of the most common recent ancestor of the Balkan RABV to be 1885 (95% HPD, 1852–1913), and skyline plots suggested an expansion of the local viral population in 1980–1990, which coincides with the observed emergence of fox rabies in the region. }, keywords = {Rabies, rabies virus (RABV), Rabv}, pubstate = {published}, tppubtype = {article} } Molecular studies of European classical rabies viruses (RABV) have revealed a number of geographically clustered lineages. To study the diversity of Balkan RABV, partial nucleoprotein (N) gene sequences were analysed from a unique panel of isolates (n5210), collected from various hosts between 1972 and 2006. All of the Balkan isolates grouped within the European/Middle East Lineage, with the majority most closely related to East European strains. A number of RABV from Bosnia & Herzegovina and Montenegro, collected between 1986 and 2006, grouped with the West European strains, believed to be responsible for the rabies epizootic that spread throughout Europe in the latter half of the 20th Century. In contrast, no Serbian RABV belonged to this sublineage. However, a distinct group of Serbian fox RABV provided further evidence for the southwards wildlife-mediated movement of rabies from Hungary, Romania and Serbia into Bulgaria. To determine the optimal region for evolutionary analysis, partial, full and concatenated N-gene and glycoprotein (G) gene sequences were compared. Whilst both the divergence times and evolutionary rates were similar irrespective of genomic region, the 95% highest probability density (HPD) limits were significantly reduced for full N-gene and concatenated NG-gene sequences compared with partial gene sequences. Bayesian coalescent analysis estimated the date of the most common recent ancestor of the Balkan RABV to be 1885 (95% HPD, 1852–1913), and skyline plots suggested an expansion of the local viral population in 1980–1990, which coincides with the observed emergence of fox rabies in the region. |
2010 |
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| Aylan, Orhan; El-Sayed, Aly Fahmy Mohamed; Farahtaj, Firouzeh; Janani, Ali R; Lugach, Olga; Tarkhan-Mouravi, Olgha; Usluer, Gaye; Vodopija, Rodovan; Vranješ, Nenad; Tordo, Noel; Dodet, Betty Report of the First Meeting of the Middle East and Eastern Europe Rabies Expert Bureau, Istanbul, Turkey Journal Article SAGE-Hindawi Access to Research, Advances in Preventive Medicine, 2011 (812515), pp. 4, 2010. Abstract | Links | BibTeX | Tags: Rabies @article{article812515, title = {Report of the First Meeting of the Middle East and Eastern Europe Rabies Expert Bureau, Istanbul, Turkey}, author = {Orhan Aylan and Aly Fahmy Mohamed El-Sayed and Firouzeh Farahtaj and Ali R. Janani and Olga Lugach and Olgha Tarkhan-Mouravi and Gaye Usluer and Rodovan Vodopija and Nenad Vranješ and Noel Tordo and Betty Dodet}, url = {https://www.pasterovzavod.rs/wp-content/uploads/2019/02/aylan2011.pdf}, doi = {10.4061/2011/812515}, year = {2010}, date = {2010-06-08}, journal = {SAGE-Hindawi Access to Research, Advances in Preventive Medicine}, volume = {2011}, number = {812515}, pages = {4}, abstract = {Rabies is a threat in all parts of the world where animal reservoirs persists, including Eastern Europe and the Middle East. Rabies experts from seven Middle East and Eastern European countries (Croatia, Egypt, Georgia, Iran, Serbia, Turkey, and Ukraine) met for two days in Istanbul, Turkey (June 8-9, 2010), to exchange information on the epidemiological situation concerning human and animal rabies in their respective countries and to discuss strategies for rabies elimination and control. They decided to establish a regional network, the Middle East and Eastern Europe Rabies Expert Bureau (MEEREB), a regional network of experts, to increase collaboration in rabies prevention and control at the local, regional, and global levels.}, keywords = {Rabies}, pubstate = {published}, tppubtype = {article} } Rabies is a threat in all parts of the world where animal reservoirs persists, including Eastern Europe and the Middle East. Rabies experts from seven Middle East and Eastern European countries (Croatia, Egypt, Georgia, Iran, Serbia, Turkey, and Ukraine) met for two days in Istanbul, Turkey (June 8-9, 2010), to exchange information on the epidemiological situation concerning human and animal rabies in their respective countries and to discuss strategies for rabies elimination and control. They decided to establish a regional network, the Middle East and Eastern Europe Rabies Expert Bureau (MEEREB), a regional network of experts, to increase collaboration in rabies prevention and control at the local, regional, and global levels. |
| Lalošević, Dušan; Lalošević, Vesna; Stojšić-Milosavljević, Anastazija; Stojšić, Đurica Borrelia-like organism in heart capillaries of patient with Lyme-disease seen by electron microscopy Journal Article International Journal of Cardiology, 145 (3), pp. e96–e98, 2010. Abstract | Links | BibTeX | Tags: Borrelia, Clinical case, Endomyocardial biopsy, Lyme borreliosis, Ultrastructure @article{article, title = {Borrelia-like organism in heart capillaries of patient with Lyme-disease seen by electron microscopy}, author = {Dušan Lalošević and Vesna Lalošević and Anastazija Stojšić-Milosavljević and Đurica Stojšić}, url = {https://www.pasterovzavod.rs/wp-content/uploads/2019/02/lalosevic2010.pdf}, doi = {https://doi.org/10.1016/j.ijcard.2008.12.165}, year = {2010}, date = {2010-01-24}, journal = {International Journal of Cardiology}, volume = {145}, number = {3}, pages = {e96–e98}, abstract = {A case of a patient who developed an acute myocarditis due to Lyme disease is reported. An increased serum antibody titer to Borrelia burgdorferi suggested a diagnosis and in addition of basic clinical methods, endomyocardial biopsy performed and analyzed by transmission electron microscopy. The lumen of myocardial capillaries was founded mostly filled with detritus and fibrin precipitate, between them several bacterial fragments were identified. The electron-microscopic characteristics of the microorganisms in this specimen, revealing irregularly coiled appearance and consistent thickness of 0.2 μm, correspond to the spiral-like structure of Lyme disease borrelia. The presence of fibrin deposits on the capillary endothelium and necrosis of myocardiocytes, suggests that the cardiopathy in our patient was represent borrelia-mediated damage of the hearth microcirculation.}, keywords = {Borrelia, Clinical case, Endomyocardial biopsy, Lyme borreliosis, Ultrastructure}, pubstate = {published}, tppubtype = {article} } A case of a patient who developed an acute myocarditis due to Lyme disease is reported. An increased serum antibody titer to Borrelia burgdorferi suggested a diagnosis and in addition of basic clinical methods, endomyocardial biopsy performed and analyzed by transmission electron microscopy. The lumen of myocardial capillaries was founded mostly filled with detritus and fibrin precipitate, between them several bacterial fragments were identified. The electron-microscopic characteristics of the microorganisms in this specimen, revealing irregularly coiled appearance and consistent thickness of 0.2 μm, correspond to the spiral-like structure of Lyme disease borrelia. The presence of fibrin deposits on the capillary endothelium and necrosis of myocardiocytes, suggests that the cardiopathy in our patient was represent borrelia-mediated damage of the hearth microcirculation. |
2008 |
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| Lalošević, Dušan; Lalošević, Vesna; Lazarević-Ivanc, Lj; Knežević, Ivana BHK-21 cell culture rabies vaccine: Lmmunogenicity of a candidate vaccine for humans Journal Article Developments in biologicals, 131 , pp. 421-9, 2008. Abstract | Links | BibTeX | Tags: BHK-21/C13, Rabies @article{article, title = {BHK-21 cell culture rabies vaccine: Lmmunogenicity of a candidate vaccine for humans}, author = {Dušan Lalošević and Vesna Lalošević and Lj Lazarević-Ivanc and Ivana Knežević}, url = {https://www.researchgate.net/publication/51409017_BHK-21_cell_culture_rabies_vaccine_Lmmunogenicity_of_a_candidate_vaccine_for_humans}, year = {2008}, date = {2008-01-00}, journal = {Developments in biologicals}, volume = {131}, pages = {421-9}, publisher = {https://www.researchgate.net/}, abstract = {Veterinary rabies vaccines produced in BHK-21/C13 permanent cell cultures have been used for a long period of time and have been proven as efficacious and safe. A candidate vaccine for human use (YU BHK Rabivak) was developed at the Pasteur Institute, Novi Sad, Serbia on the basis of the fixed rabies virus strain "L. Pasteur 2061/Vero 15 pas" using BHK 21/C13 as a cell substrate for vaccine production. To test the vaccine immunogenicity, a clinical trial was conducted involving 164 subjects between 18 and 60 years of age, immunized either with the YU BHK Rabivak vaccine candidate orwith a commercially available vaccine (Rabipur). Three groups of subjects were immunized with either vaccine by intramuscular administration in the deltoid region, following a pre-exposure regimen on days 0, 7 and 21, or the Essen or Zagreb post-exposure regimens. Rabies virus neutralizing antibodies (VNA) titres were determined by rapid fluorescent focus inhibition test (RFFIT) 21 and 30-45 days post vaccination. A protective titre of VNAs (>0.5 IU/ml) was found in all subjects vaccinated. Dynamics of the immune response showed that 96.4% of the subjects developed protective VNA titres after two doses, 99.3% after three doses and 100% after four and five doses of the candidate YU BHK Rabivak vaccine. There was a low reactogenicity without serious adverse events indicating a satisfactory safety profile in humans. Results obtained in this study indicate that BHK 21 cells offer the possibility of producing an efficacious and safe cell-culture rabies vaccine for humane use.}, keywords = {BHK-21/C13, Rabies}, pubstate = {published}, tppubtype = {article} } Veterinary rabies vaccines produced in BHK-21/C13 permanent cell cultures have been used for a long period of time and have been proven as efficacious and safe. A candidate vaccine for human use (YU BHK Rabivak) was developed at the Pasteur Institute, Novi Sad, Serbia on the basis of the fixed rabies virus strain "L. Pasteur 2061/Vero 15 pas" using BHK 21/C13 as a cell substrate for vaccine production. To test the vaccine immunogenicity, a clinical trial was conducted involving 164 subjects between 18 and 60 years of age, immunized either with the YU BHK Rabivak vaccine candidate orwith a commercially available vaccine (Rabipur). Three groups of subjects were immunized with either vaccine by intramuscular administration in the deltoid region, following a pre-exposure regimen on days 0, 7 and 21, or the Essen or Zagreb post-exposure regimens. Rabies virus neutralizing antibodies (VNA) titres were determined by rapid fluorescent focus inhibition test (RFFIT) 21 and 30-45 days post vaccination. A protective titre of VNAs (>0.5 IU/ml) was found in all subjects vaccinated. Dynamics of the immune response showed that 96.4% of the subjects developed protective VNA titres after two doses, 99.3% after three doses and 100% after four and five doses of the candidate YU BHK Rabivak vaccine. There was a low reactogenicity without serious adverse events indicating a satisfactory safety profile in humans. Results obtained in this study indicate that BHK 21 cells offer the possibility of producing an efficacious and safe cell-culture rabies vaccine for humane use. |