Публикације и научни радови Пастеровог завода…
ПУБЛИКАЦИЈЕ
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![]() | Cimpean, Anca Maria; Lalošević, Dušan; Lalošević, Vesna; BANOVIĆ, PAVLE; Raica, Marius; Mederle, Ovidiu Alexandru In Vivo, 32 (4), pp. 791-798, 2018. Садржај | Линкови | BibTeX | Ознаке: BHK 21/C13 cell line, chick embryo chorioallantoic membrane, experimental therapy, phenotype characterization @article{cimpean2018, title = {Disodium Cromolyn and Anti-podoplanin Antibodies Strongly Inhibit Growth of BHK 21/C13-derived Fibrosarcoma in a Chick Embryo Chorioallantoic Membrane Model }, author = {Anca Maria Cimpean and Dušan Lalošević and Vesna Lalošević and PAVLE BANOVIĆ and Marius Raica and Ovidiu Alexandru Mederle}, url = {https://www.pasterovzavod.rs/wp-content/uploads/2019/02/cimpean2018.pdf}, doi = {10.21873/invivo.11309}, year = {2018}, date = {2018-03-04}, journal = {In Vivo}, volume = {32}, number = {4}, pages = {791-798}, institution = {Department of Microscopic Morphology/Histology, Angiogenesis Research Center, Victor Babes University of Medicine and Pharmacy, Timisoara, Romania; Department of Histology and Embryology, Medical Faculty, and Department of Veterinary Medicine, Faculty of Agriculture, University of Novi Sad, Novi Sad, Serbia}, abstract = {AIM: To characterize baby hamster kidney fibroblast (BHK 21/C13) cells and test the effects of antibodies against podoplanin and disodium cromolyn on BHK 21/C13 cell line-derived tumors grown on chick embryo chorioallantoic membrane (CAM). MATERIAL AND METHODS: BHK 21/C13 cell-derived fibrosarcomas developed in hamsters were implanted on CAM and treated with anti-podoplanin antibodies and disodium cromolyn. BHK 21/C13 cell immunophenotype was assessed. RESULTS: Fibrosarcoma cells were positive for vimentin, CD117, smooth muscle actin, vascular endothelial growth factor epidermal growth factor receptor, homebox prospero gene 1 and negative for platelet-derived growth factor B, neuron-specific enolase, S100, CD34, Ewing sarcoma and podoplanin. CAM-grown fibrosarcomas were highly sensitive to disodium cromolyn and anti-podoplanin antibodies. CONCLUSION: Immunophenotyping BHK 21/C13 cells and their response to drugs represent the first step in revealing cell line utility and a reliable tool for experimental cancer research.}, keywords = {BHK 21/C13 cell line, chick embryo chorioallantoic membrane, experimental therapy, phenotype characterization}, pubstate = {published}, tppubtype = {article} } AIM: To characterize baby hamster kidney fibroblast (BHK 21/C13) cells and test the effects of antibodies against podoplanin and disodium cromolyn on BHK 21/C13 cell line-derived tumors grown on chick embryo chorioallantoic membrane (CAM). MATERIAL AND METHODS: BHK 21/C13 cell-derived fibrosarcomas developed in hamsters were implanted on CAM and treated with anti-podoplanin antibodies and disodium cromolyn. BHK 21/C13 cell immunophenotype was assessed. RESULTS: Fibrosarcoma cells were positive for vimentin, CD117, smooth muscle actin, vascular endothelial growth factor epidermal growth factor receptor, homebox prospero gene 1 and negative for platelet-derived growth factor B, neuron-specific enolase, S100, CD34, Ewing sarcoma and podoplanin. CAM-grown fibrosarcomas were highly sensitive to disodium cromolyn and anti-podoplanin antibodies. CONCLUSION: Immunophenotyping BHK 21/C13 cells and their response to drugs represent the first step in revealing cell line utility and a reliable tool for experimental cancer research. |