@article{cimpean2018,
title = {Disodium Cromolyn and Anti-podoplanin Antibodies Strongly Inhibit Growth of BHK 21/C13-derived Fibrosarcoma in a Chick Embryo Chorioallantoic Membrane Model },
author = {Anca Maria Cimpean and Dušan Lalošević and Vesna Lalošević and PAVLE BANOVIĆ and Marius Raica and Ovidiu Alexandru Mederle},
url = {https://www.pasterovzavod.rs/wp-content/uploads/2019/02/cimpean2018.pdf},
doi = {10.21873/invivo.11309},
year = {2018},
date = {2018-03-04},
journal = {In Vivo},
volume = {32},
number = {4},
pages = {791-798},
institution = {Department of Microscopic Morphology/Histology, Angiogenesis Research Center, Victor Babes University of Medicine and Pharmacy, Timisoara, Romania; Department of Histology and Embryology, Medical Faculty, and Department of Veterinary Medicine, Faculty of Agriculture, University of Novi Sad, Novi Sad, Serbia},
abstract = {AIM:
To characterize baby hamster kidney fibroblast (BHK 21/C13) cells and test the effects of antibodies against podoplanin and disodium cromolyn on BHK 21/C13 cell line-derived tumors grown on chick embryo chorioallantoic membrane (CAM).

MATERIAL AND METHODS:
BHK 21/C13 cell-derived fibrosarcomas developed in hamsters were implanted on CAM and treated with anti-podoplanin antibodies and disodium cromolyn. BHK 21/C13 cell immunophenotype was assessed.

RESULTS:
Fibrosarcoma cells were positive for vimentin, CD117, smooth muscle actin, vascular endothelial growth factor epidermal growth factor receptor, homebox prospero gene 1 and negative for platelet-derived growth factor B, neuron-specific enolase, S100, CD34, Ewing sarcoma and podoplanin. CAM-grown fibrosarcomas were highly sensitive to disodium cromolyn and anti-podoplanin antibodies.

CONCLUSION:
Immunophenotyping BHK 21/C13 cells and their response to drugs represent the first step in revealing cell line utility and a reliable tool for experimental cancer research.},
keywords = {BHK 21/C13 cell line, chick embryo chorioallantoic membrane, experimental therapy, phenotype characterization},
pubstate = {published},
tppubtype = {article}
}