@article{Banović2022,

title = {Clinical Aspects and Detection of Emerging Rickettsial Pathogens: A “One Health” Approach Study in Serbia, 2020},

author = {Pavle Banović and Adrian Alberto Díaz-Sánchez and Verica Simin and Angelique Foucault-Simonin and Clemence Galon and Alejandra Wu Chuang and Dragana Mijatović and Dasiel Obregon and Sara Moutailler and Alejandro Cabezas-Cruz},

editor = {Antonio Battisti

},

url = {https://www.frontiersin.org/articles/10.3389/fmicb.2021.797399/full},

doi = {doi.org/10.3389/fmicb.2021.797399},

year  = {2022},

date = {2022-01-26},

journal = {Frontiers in Microbiology},

abstract = {Ticks carry numerous pathogens that, if transmitted, can cause disease in susceptible humans and animals. The present study describes our approach on how to investigate clinical presentations following tick bites in humans. To this aim, the occurrence of major tick-borne pathogens (TBPs) in human blood samples (n = 85) and the ticks collected (n = 93) from the same individuals were tested using an unbiased high-throughput pathogen detection microfluidic system. The clinical symptoms were characterized in enrolled patients. In patients with suspected TBP infection, serological assays were conducted to test for the presence of antibodies against specific TBPs. A field study based on One Health tenets was further designed to identify components of a potential chain of infection resulting in Rickettsia felis infection in one of the patients. Ticks species infesting humans were identified as Ixodes ricinus, Rhipicephalus sanguineus sensu lato (s.l.), Dermacentor reticulatus, and Haemaphysalis punctata. Five patients developed local skin lesions at the site of the tick bite including erythema migrans, local non-specific reactions, and cutaneous hypersensitivity reaction. Although Borrelia burgdorferi s.l., Babesia microti, Anaplasma phagocytophilum, and Candidatus Cryptoplasma sp. DNAs were detected in tick samples, different Rickettsia species were the most common TBPs identified in the ticks. The presence of TBPs such as Rickettsia helvetica, Rickettsia monacensis, Borrelia lusitaniae, Borrelia burgdorferi, Borrelia afzelii, A. phagocytophilum, and B. microti in ticks was further confirmed by DNA sequencing. Two of the patients with local skin lesions had IgG reactive against spotted fever group rickettsiae, while IgM specific to B. afzelii, Borrelia garinii, and Borrelia spielmanii were detected in the patient with erythema migrans. Although R. felis infection was detected in one human blood sample, none of the components of the potential chain of infection considered in this study tested positive to this pathogen either using direct pathogen detection in domestic dogs or xenodiagnosis in ticks collected from domestic cats. The combination of high-throughput screening of TBPs and One Health approaches might help characterize chains of infection leading to human infection by TBPs, as well as prevalence of emerging rickettsial pathogens in the Balkan region.},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{Smieško2022,

title = {What is Fishy in Asymptomatic Patients? Co-Occurrence of Aerococcus urinae Infection in Pediatric Patient with Phimosis},

author = {Gordana Smieško and Pavle Banović and Vera Gusman and Biljana Milosavljević and Deana Medić and Tamara Bulajić and Milica Pejaković Budinski and Svetlana Golocorbin-Kon},

url = {https://publisher.medfak.ni.ac.rs/AFMN_1/2021/4-2021/full%20text/10What%20is%20Fishy%20G.Smiesko.pdf},

doi = {10.5937/afmnai38-32051},

year  = {2022},

date = {2022-01-12},

journal = {Acta Facultatis Medicae Naissensis},

volume = {38},

number = {4},

abstract = { Here we present the first case of Aerococcus urinae isolated from urine sample of a 5-year-old boy from Vojvodina province (Serbia). The patient had predisposing factors related to the susceptibility of genitourinary system to infection. Thus, he reported no complaints related to active urinary infections. Eradication of infection was achieved after 10-day treatment with meropenem.



From this case, the question emerges: Is there a possibility that A. urinae infections are neglected in pediatric patients with susceptibility of genitourinary system to infection? Only after wide introduction of matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) in competent laboratories, we will be able to give a precise answer.},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{Stankov2021,

title = {O značaju higijenskih mera u suzbijanju infektivnih bolesti koje se prenose respiratornim putem},

author = {Srđan Stankov},

doi = {https://doi.org/10.5937/smclk2-32327},

year  = {2021},

date = {2021-06-23},

journal = {Srpski medicinski časopis Lekarske komore},

volume = {2},

number = {1},

pages = {11-15},

publisher = {Lekarska komora},

abstract = {Zarazne bolesti su postojale u obliku epidemija tokom istorije čovečanstva, a patogeni mikroorganizmi su dugo bili smatrani odgovornim za njihovo pojavljivanje. Međutim, takozvani biološki agensi akutnih zaraznih bolesti nisu njihovi stvarni uzroci, već samo katalizatori već postojećih patoloških procesa. Otuda ekstremna varijabilnost lokacije i intenziteta patoloških procesa sa istim biološkim uzročnikom kod različitih jedinki vrste domaćina. Patogenost i virulencija mikroorganizama nisu njihova vlastita i nepromenljiva svojstva, već su samo rezultat patogenosti neživih faktora životne sredine koji deluju na domaćina i koji obično ostaju van fokusa medicinskih istraživanja. Pitanje od glavne praktične važnosti, kako da sprečimo pojavu zaraznih bolesti, daje nam istorija. U prošlosti su mnoge higijenske mere bile dovoljne da se većina zaraznih bolesti svede na neznatnu pojavu. Do sada je društvo puno uradilo na higijenskim standardima čvrstih i tečnih materija u našem okruženju. Međutim, briga o higijeni atmosfere i dalje je prilično nerazvijena i zanemarena. Danas se atmosfera neprekidno i sistematski zagađuje određenim gasovima, parama kao i česticama. Direktni rezultat ovakog stanja razvoja higijenske prakse je da su danas jedina vrsta epidemija koje pogađaju opštu populaciju epidemije koje se šire atmosferom, tj. respiratorne epidemije. Da bi se trajno eliminisala dalja mogućnost respiratornih epidemija, neophodno je uvesti trajne i sistematske mere za održavanje higijene atmosfere ljudskih naselja.},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{Banović2021c,

title = {A One Health approach to study the circulation of tick-borne pathogens: A preliminary study},

author = {Pavle Banović and Adrian Alberto Díaz-Sánchez and Clemence Galon and Angélique Foucault-Simonin and Verica Simin and Dragana Mijatović and Luka Papić and Alejandra Wu-Chuang and Dasiel Obregon and Sara Moutailler and Alejandro Cabezas-Cruz },

doi = {https://doi.org/10.1016/j.onehlt.2021.100270},

year  = {2021},

date = {2021-06-01},

journal = {One Health},

abstract = {Tick-borne pathogens (TBPs) have complex life cycles involving tick vectors and vertebrate hosts. However, there is limited empirical evidence on the zoonotic circulation of TBPs. In this study, we used a One Health approach to study the possible circulation of TBPs in ticks, animals and humans within a rural household in the foothills of the Fruška Gora mountain, northern Serbia. The presence of TBP DNA was assessed using microfluidic PCR (25 bacterial species, 7 parasite species, 5 bacterial genera, 3 parasite genera) in animal, human and tick samples and the presence of tick-borne encephalitis virus (TBEV) RNA was screened for using RT-qPCR on tick samples. In addition, Lyme borreliosis serology was assessed in patient sera. Rhipicephalus sanguineus and Ixodes ricinus ticks were identified on dogs and Haemaphysalis punctata was identified on house walls. Rickettsia helvetica was the most common pathogen detected in pooled R. sanguineus and I. ricinus tick samples, followed by Hepatozoon canis. None of the H. punctata tick samples tested positive for the presence of TBPs. Anaplasma phagocytophilum and Rickettsia monacensis were the most frequent pathogens detected in dogs, followed by Rickettsia felis, whereas Anaplasma bovis was the only pathogen found in one of the goats tested. None of the human blood samples collected from family members tested positive for the presence of TBPs. Although microfluidic PCR did not detect Borrelia sp. in any of the tested tick or blood samples, a family member with a history of Lyme disease was seropositive for Borrelia burgdorferi sensu lato (s.l.). We conclude that, despite the presence of TBPs in tick and vertebrate reservoirs, there is no evidence of infection with TBPs across various components of the epidemiological chain in a rural Fruška Gora household.},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{Ristić2021,

title = {Uticaj hroničnog imobilizacionog stresa na estrusni ciklus kod ženki miševa soja NMRI},

author = {Jelena Ristić and Damir Bogdan and Pavle Banović},

doi = {10.5937/mp72-28778},

year  = {2021},

date = {2021-05-20},

journal = {Medicinski podmladak},

volume = {72},

number = {1},

pages = {6-11},

abstract = {Uvod: Stres je skup reakcija organizma koje nastaju usled dejstva faktora spoljašnje sredine. Da bi se održala homeostaza i zaštitio organizam, uključuju se brojni adaptacioni mehanizmi. Stresori koji deluju trenutno ili kratkoročno izazivaju akutni stres koji ima protektivni efekat. Kada stresor deluje duže, a organizam ne uspeva da odgovori na izazov, javlja se hronični stres koji vodi u patološko stanje. Hronični stres je, kao etiološki faktor, doveden u vezu sa poremećajima menstrualnog ciklusa žena. Cilj: Cilj je ispitati dejstvo hroničnog imobilizacionog stresa na estrusni ciklus kod ženki miševa NMRI soja. Materijal i metode: Dvanaest polno zrelih ženki miševa NMRI soja nasumično je podeljeno u kontrolnu (n = 6) i eksperimentalnu grupu (n = 6). Eksperimentalnoj grupi je indukovan hronični stres ubacivanjem u imobilizacione komore 2 sata dnevno u periodu od 14 dana. Svakodnevno je svim životinjama uziman vaginalni ispirak od koga je pravljen razmaz radi ispitivanja pod svetlosnim mikroskopom. Analiza preparata je obuhvatala određivanje faza estrusnog ciklusa. Za analizu efekta stresa statistički je ispitana učestalost smenjivanja estrusnih faza u eksperimentalnoj grupi naspram kontrolne. Rezultati: Kod ženki miševa je uočen estrusni ciklus koji se sastoji iz četiri faze: proestrusa, estrusa, metestrusa i diestrusa. U adaptacionom periodu je zapažen prolongirani diestrus, dominantno prisutan u obe grupe. Kod stresiranih jedinki je uočen izlazak iz diestrusa i pojava drugih estrusnih faza, za razliku od kontrolnih kod kojih je diesterus bio znatno učestaliji. Zapažene su neregularnosti u trajanju i smenjivanju faza kod jedinki eksperimentalne grupe, gde se učestalost odstupanja od diestrusa pokazala kao statistički značajna (p < 0,01). Zaključak: Indukovanje imobilizacionog stresa dovodi do izlaska životinja iz diestrusa i velikih varijacija u trajanju i smenjivanju faza estrusnog ciklusa ženki miševa soja NMRI. S obzirom na to da je hormonski mehanizam regulacije estrusnog ciklusa kod ženki miševa soja NMRI sličan mehanizmu regulacije menstrualnog ciklusa kod žena, mogu poslužiti kao dobar model za proučavanje reproduktivnih poremećaja kod žena.},

keywords = {},

pubstate = {published},

tppubtype = {article}

}