 | Fedrigolli, Elsa; Bogdan, Damir; Lalošević, Dušan; Banović, Pavle Proposition Of A Simplified Protocol and New Parameter Introduction In NMRI Mice Anhedonia Induction Journal Article Forthcoming Serbian Journal of Experimental and Clinical Research, In press , Forthcoming. BibTeX @article{Fedrigolli2020,
title = {Proposition Of A Simplified Protocol and New Parameter Introduction In NMRI Mice Anhedonia Induction},
author = {Elsa Fedrigolli and Damir Bogdan and Dušan Lalošević and Pavle Banović},
year = {2020},
date = {2020-04-30},
journal = {Serbian Journal of Experimental and Clinical Research},
volume = {In press},
keywords = {},
pubstate = {forthcoming},
tppubtype = {article}
}
|
| Stankov, Srđan; Obradović, Nemanja; Vujin, Dragana; Vranješ, Nenad Possibility of Immediate Introduction of a Single-Dose Antibody Induction Test as a Refinement of the NIH Test for Inactivated Rabies Vaccine Potency Determination Journal Article Forthcoming Viral Immunology, pp. 1-11, Forthcoming. Sadržaj | Linkovi | BibTeX @article{Stankov2020,
title = {Possibility of Immediate Introduction of a Single-Dose Antibody Induction Test as a Refinement of the NIH Test for Inactivated Rabies Vaccine Potency Determination},
author = {Srđan Stankov and Nemanja Obradović and Dragana Vujin and Nenad Vranješ},
url = {https://www.liebertpub.com/doi/10.1089/vim.2019.0131},
doi = {10.1089/vim.2019.0131},
year = {2020},
date = {2020-03-10},
journal = {Viral Immunology},
pages = {1-11},
abstract = {Antibody induction test (AIT) is a promising candidate as a refinement of the troublesome National institutes of Health (NIH) test in the sense of animal welfare 3R approach for determination of potency of inactivated rabies vaccines for veterinary and human use. In this study, we initially try to develop AIT as a suitable alternative to NIH test, to achieve a reduction of test duration and diminish animal suffering by omitting intracerebral CVS infection and measuring humoral immunity upon vaccination. Designs of both multi-dose and single-dose AIT were examined. Biological reference preparation, batch 5 with assigned titer of 10 IU/vial, was taken as both standard and test vaccine. Six consecutive AITs were performed and eight pools of sera in each AIT were tested in triplicate by rapid fluorescent focus inhibition test. We estimated the upper detection limit and calculated test variability for individual dilutions. For multi-dose AIT, we estimated the dose–response function and performed calculations of final test results and statistical validity parameters for both linear and sigmoidal model using CombiStats program. Sigmoidal 4-parameter dose–response model was found optimal. Presented design of
multi-dose AIT showed a satisfactory detection limit for testing of inactivated rabies vaccines for both veterinary and human use. However, due to nonconformity of obtained results with statistical validity criteria, we concluded that the presented model of multi-dose AIT was unsuitable for introduction in routine practice.
However, we concluded that there was a realistic option for introduction of two versions of single-dose AIT. The first version would be with two standard vaccine controls and could be introduced immediately, while the second version would include testing of the sample only and rely on comparison of the induced rabies antibody level with absolute cut-off limits set in advance.},
keywords = {},
pubstate = {forthcoming},
tppubtype = {article}
}
Antibody induction test (AIT) is a promising candidate as a refinement of the troublesome National institutes of Health (NIH) test in the sense of animal welfare 3R approach for determination of potency of inactivated rabies vaccines for veterinary and human use. In this study, we initially try to develop AIT as a suitable alternative to NIH test, to achieve a reduction of test duration and diminish animal suffering by omitting intracerebral CVS infection and measuring humoral immunity upon vaccination. Designs of both multi-dose and single-dose AIT were examined. Biological reference preparation, batch 5 with assigned titer of 10 IU/vial, was taken as both standard and test vaccine. Six consecutive AITs were performed and eight pools of sera in each AIT were tested in triplicate by rapid fluorescent focus inhibition test. We estimated the upper detection limit and calculated test variability for individual dilutions. For multi-dose AIT, we estimated the dose–response function and performed calculations of final test results and statistical validity parameters for both linear and sigmoidal model using CombiStats program. Sigmoidal 4-parameter dose–response model was found optimal. Presented design of
multi-dose AIT showed a satisfactory detection limit for testing of inactivated rabies vaccines for both veterinary and human use. However, due to nonconformity of obtained results with statistical validity criteria, we concluded that the presented model of multi-dose AIT was unsuitable for introduction in routine practice.
However, we concluded that there was a realistic option for introduction of two versions of single-dose AIT. The first version would be with two standard vaccine controls and could be introduced immediately, while the second version would include testing of the sample only and rely on comparison of the induced rabies antibody level with absolute cut-off limits set in advance. |
| Pavle, Banović; Čapo, Ivan; Ogorelica, Dejan; Vranješ, Nenad; Verica, Simin; Lalošević, Dušan Mysterious path of Borrelia spielmanii: spreading without morphological alteration of collagen type I and IV Journal Article Future Microbiology, 2020. Sadržaj | Linkovi | BibTeX @article{Pavle2020,
title = {Mysterious path of Borrelia spielmanii: spreading without morphological alteration of collagen type I and IV},
author = {Banović Pavle and Ivan Čapo and Dejan Ogorelica and Nenad Vranješ and Simin Verica and Dušan Lalošević},
url = {https://www.futuremedicine.com/doi/10.2217/fmb-2019-0217},
doi = {https://doi.org/10.2217/fmb-2019-0217},
year = {2020},
date = {2020-01-06},
journal = {Future Microbiology},
abstract = {The majority of suggested mechanisms of Borrelia spreading inside erythema migrans (EM) are developed from in vitro studies and animal models. This report is the first to describe pathomorphological substrate of EM caused by Borrelia spielmanii in humans, addressing the hypothesis of enhanced Borrelia penetration through extracellular matrix. In the process of ruling out of atypical Masters’ disease, we conducted a punch biopsy of suspected EM and a two-tier serology testing for Lyme borreliosis, where we registered antibodies against B. spielmanii. Skin biopsy showed CD4+ and CD8+ lymphocyte involvement and high activity of matrix metalloproteinase 9. No alterations were detected in distribution and morphology of collagen type I and IV. Therefore, it is suggested that other mechanisms should be considered as major contributing factors to local spreading of B.spielmanii.},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
The majority of suggested mechanisms of Borrelia spreading inside erythema migrans (EM) are developed from in vitro studies and animal models. This report is the first to describe pathomorphological substrate of EM caused by Borrelia spielmanii in humans, addressing the hypothesis of enhanced Borrelia penetration through extracellular matrix. In the process of ruling out of atypical Masters’ disease, we conducted a punch biopsy of suspected EM and a two-tier serology testing for Lyme borreliosis, where we registered antibodies against B. spielmanii. Skin biopsy showed CD4+ and CD8+ lymphocyte involvement and high activity of matrix metalloproteinase 9. No alterations were detected in distribution and morphology of collagen type I and IV. Therefore, it is suggested that other mechanisms should be considered as major contributing factors to local spreading of B.spielmanii. |
